PMID- 23446353
OWN - NLM
STAT- MEDLINE
DCOM- 20130326
LR  - 20240210
IS  - 1476-4687 (Electronic)
IS  - 0028-0836 (Linking)
VI  - 495
IP  - 7439
DP  - 2013 Mar 7
TI  - Intestinal label-retaining cells are secretory precursors expressing Lgr5.
PG  - 65-9
LID - 10.1038/nature11965 [doi]
AB  - The rapid cell turnover of the intestinal epithelium is achieved from small 
      numbers of stem cells located in the base of glandular crypts. These stem cells 
      have been variously described as rapidly cycling or quiescent. A functional 
      arrangement of stem cells that reconciles both of these behaviours has so far 
      been difficult to obtain. Alternative explanations for quiescent cells have been 
      that they act as a parallel or reserve population that replace rapidly cycling 
      stem cells periodically or after injury; their exact nature remains unknown. Here 
      we show mouse intestinal quiescent cells to be precursors that are committed to 
      mature into differentiated secretory cells of the Paneth and enteroendocrine 
      lineage. However, crucially we find that after intestinal injury they are capable 
      of extensive proliferation and can give rise to clones comprising the main 
      epithelial cell types. Thus, quiescent cells can be recalled to the stem-cell 
      state. These findings establish quiescent cells as an effective clonogenic 
      reserve and provide a motivation for investigating their role in pathologies such 
      as colorectal cancers and intestinal inflammation.
FAU - Buczacki, Simon J A
AU  - Buczacki SJ
AD  - Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson 
      Way, Cambridge CB2 0RE, UK.
FAU - Zecchini, Heather Ireland
AU  - Zecchini HI
FAU - Nicholson, Anna M
AU  - Nicholson AM
FAU - Russell, Roslin
AU  - Russell R
FAU - Vermeulen, Louis
AU  - Vermeulen L
FAU - Kemp, Richard
AU  - Kemp R
FAU - Winton, Douglas J
AU  - Winton DJ
LA  - eng
SI  - GEO/GSE43772
GR  - 17044/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130227
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Biomarkers)
RN  - 0 (Lgr5 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
CIN - Nature. 2013 Mar 7;495(7439):53-4. doi: 10.1038/nature11958. PMID: 23446347
CIN - Nat Rev Mol Cell Biol. 2013 Apr;14(4):193. doi: 10.1038/nrm3551. PMID: 23486280
CIN - Cell Stem Cell. 2013 Apr 4;12(4):389-90. doi: 10.1016/j.stem.2013.03.009. PMID: 
      23561439
CIN - Nat Rev Mol Cell Biol. 2013 Apr;14(4):193. PMID: 23847776
MH  - Animals
MH  - Biomarkers/analysis/metabolism
MH  - Cell Differentiation
MH  - Cell Division
MH  - *Cell Lineage
MH  - Cell Separation
MH  - Clone Cells/cytology/metabolism
MH  - Intestinal Neoplasms/pathology
MH  - Intestines/cytology/injuries/pathology
MH  - Mice
MH  - Multipotent Stem Cells/*cytology/*metabolism
MH  - Paneth Cells/*cytology/*metabolism
MH  - Receptors, G-Protein-Coupled/*metabolism
MH  - Regeneration
MH  - Staining and Labeling
MH  - Stem Cell Niche
EDAT- 2013/03/01 06:00
MHDA- 2013/03/27 06:00
CRDT- 2013/03/01 06:00
PHST- 2012/07/26 00:00 [received]
PHST- 2013/01/30 00:00 [accepted]
PHST- 2013/03/01 06:00 [entrez]
PHST- 2013/03/01 06:00 [pubmed]
PHST- 2013/03/27 06:00 [medline]
AID - nature11965 [pii]
AID - 10.1038/nature11965 [doi]
PST - ppublish
SO  - Nature. 2013 Mar 7;495(7439):65-9. doi: 10.1038/nature11965. Epub 2013 Feb 27.