PMID- 23529826
OWN - NLM
STAT- MEDLINE
DCOM- 20130617
LR  - 20131029
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 43
IP  - 5
DP  - 2013 May
TI  - Efficiently stimulated adult microglia cross-prime naive CD8+ T cells injected in 
      the brain.
PG  - 1173-84
LID - 10.1002/eji.201243040 [doi]
AB  - Microglia are the major myeloid-immune cells of the brain parenchyma. In a steady 
      state, microglia monitor their environment for pathogens or damaged cells. In 
      response to neural injury or inflammation, microglia become competent APCs able 
      to prime CD4(+) and CD8(+) T lymphocytes. We previously demonstrated that 
      neonatal and adult microglia cross-present exogenous soluble Ags in vitro. 
      However, whether microglia are able to cross-present Ag to naive CD8(+) T cells 
      in vivo, within the brain microenvironment, remains undetermined. Here, we have 
      designed an original protocol in order to exclude the involvement in 
      cross-presentation activity of peripheral migrating APCs and of CNS-associated 
      APCs. In C57Bl/6 mice, in which the body but not the head has been properly 
      irradiated, we analyzed the ability of resident microglia to stimulate 
      intracerebrally injected CD8(+) T cells in vivo. This study demonstrates for the 
      first time that adult microglia cross-present Ag to naive CD8(+) T cells in vivo 
      and that full microglia activation is required to overcome the inhibitory 
      constrains of the brain and to render microglia able to cross-prime naive CD8(+) 
      T cells injected in the brain. These observations offer new insights in 
      brain-tumor immunotherapy based on the induction of cytotoxic antitumoral T 
      cells.
CI  - (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Jarry, Ulrich
AU  - Jarry U
AD  - L'UNAM Universite, Universite d'Angers, Angers, France.
FAU - Jeannin, Pascale
AU  - Jeannin P
FAU - Pineau, Laurent
AU  - Pineau L
FAU - Donnou, Sabrina
AU  - Donnou S
FAU - Delneste, Yves
AU  - Delneste Y
FAU - Couez, Dominique
AU  - Couez D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130409
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigen-Presenting Cells/cytology/*immunology
MH  - Antigens/immunology
MH  - Brain/cytology/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology/transplantation
MH  - *Cross-Priming
MH  - Gamma Rays
MH  - Injections, Intraventricular
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/cytology/*immunology
MH  - Solubility
EDAT- 2013/03/27 06:00
MHDA- 2013/06/19 06:00
CRDT- 2013/03/27 06:00
PHST- 2012/10/02 00:00 [received]
PHST- 2013/01/31 00:00 [revised]
PHST- 2013/02/21 00:00 [accepted]
PHST- 2013/03/27 06:00 [entrez]
PHST- 2013/03/27 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
AID - 10.1002/eji.201243040 [doi]
PST - ppublish
SO  - Eur J Immunol. 2013 May;43(5):1173-84. doi: 10.1002/eji.201243040. Epub 2013 Apr 
      9.