PMID- 23530069
OWN - NLM
STAT- MEDLINE
DCOM- 20130523
LR  - 20211021
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 201
IP  - 1
DP  - 2013 Apr 1
TI  - FANCJ couples replication past natural fork barriers with maintenance of 
      chromatin structure.
PG  - 33-48
LID - 10.1083/jcb.201208009 [doi]
AB  - Defective DNA repair causes Fanconi anemia (FA), a rare childhood 
      cancer-predisposing syndrome. At least 15 genes are known to be mutated in FA; 
      however, their role in DNA repair remains unclear. Here, we show that the FANCJ 
      helicase promotes DNA replication in trans by counteracting fork stalling on 
      replication barriers, such as G4 quadruplex structures. Accordingly, 
      stabilization of G4 quadruplexes in DeltaFANCJ cells restricts fork movements, 
      uncouples leading- and lagging-strand synthesis and generates small 
      single-stranded DNA gaps behind the fork. Unexpectedly, we also discovered that 
      FANCJ suppresses heterochromatin spreading by coupling fork movement through 
      replication barriers with maintenance of chromatin structure. We propose that 
      FANCJ plays an essential role in counteracting chromatin compaction associated 
      with unscheduled replication fork stalling and restart, and suppresses 
      tumorigenesis, at least partially, in this replication-specific manner.
FAU - Schwab, Rebekka A
AU  - Schwab RA
AD  - The Weatherall Institute of Molecular Medicine, Oxford University, Oxford, 
      England, UK.
FAU - Nieminuszczy, Jadwiga
AU  - Nieminuszczy J
FAU - Shin-ya, Kazuo
AU  - Shin-ya K
FAU - Niedzwiedz, Wojciech
AU  - Niedzwiedz W
LA  - eng
GR  - 07-0922/AICR_/Worldwide Cancer Research/United Kingdom
GR  - G0902418/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130325
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Avian Proteins)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Heterochromatin)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
MH  - Animals
MH  - Avian Proteins/genetics/*metabolism
MH  - Cell Line
MH  - Chickens
MH  - DNA Helicases/genetics/*metabolism
MH  - DNA Repair/*physiology
MH  - DNA Replication/*physiology
MH  - DNA, Single-Stranded/genetics/*metabolism
MH  - Fanconi Anemia/genetics/metabolism
MH  - Heterochromatin/*enzymology/genetics
MH  - Humans
PMC - PMC3613694
EDAT- 2013/03/27 06:00
MHDA- 2013/05/25 06:00
PMCR- 2013/10/01
CRDT- 2013/03/27 06:00
PHST- 2013/03/27 06:00 [entrez]
PHST- 2013/03/27 06:00 [pubmed]
PHST- 2013/05/25 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - jcb.201208009 [pii]
AID - 201208009 [pii]
AID - 10.1083/jcb.201208009 [doi]
PST - ppublish
SO  - J Cell Biol. 2013 Apr 1;201(1):33-48. doi: 10.1083/jcb.201208009. Epub 2013 Mar 
      25.