PMID- 23553902
OWN - NLM
STAT- MEDLINE
DCOM- 20140507
LR  - 20230603
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 31
IP  - 9
DP  - 2013 Sep
TI  - Brief report: CD24 and CD44 mark human intestinal epithelial cell populations 
      with characteristics of active and facultative stem cells.
PG  - 2024-30
LID - 10.1002/stem.1391 [doi]
AB  - Recent seminal studies have rapidly advanced the understanding of intestinal 
      epithelial stem cell (IESC) biology in murine models. However, the lack of 
      techniques suitable for isolation and subsequent downstream analysis of IESCs 
      from human tissue has hindered the application of these findings toward the 
      development of novel diagnostics and therapies with direct clinical relevance. 
      This study demonstrates that the cluster of differentiation genes CD24 and CD44 
      are differentially expressed across LGR5 positive "active" stem cells as well as 
      HOPX positive "facultative" stem cells. Fluorescence-activated cell sorting 
      enables differential enrichment of LGR5 (CD24-/CD44+) and HOPX (CD24+/CD44+) 
      cells for gene expression analysis and culture. These findings provide the 
      fundamental methodology and basic cell surface signature necessary for isolating 
      and studying intestinal stem cell populations in human physiology and disease.
CI  - (c) AlphaMed Press.
FAU - Gracz, Adam D
AU  - Gracz AD
AD  - Department of Medicine Division of Gastroenterology and Hepatology, The 
      University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA; 
      Department of Cell Biology and Physiology, The University of North Carolina at 
      Chapel Hill, Chapel Hill, North Carolina, USA.
FAU - Fuller, Megan K
AU  - Fuller MK
FAU - Wang, Fengchao
AU  - Wang F
FAU - Li, Linheng
AU  - Li L
FAU - Stelzner, Matthias
AU  - Stelzner M
FAU - Dunn, James C Y
AU  - Dunn JC
FAU - Martin, Martin G
AU  - Martin MG
FAU - Magness, Scott T
AU  - Magness ST
LA  - eng
GR  - U01 DK085535/DK/NIDDK NIH HHS/United States
GR  - P30 DK034987/DK/NIDDK NIH HHS/United States
GR  - U24 DK085532/DK/NIDDK NIH HHS/United States
GR  - P30 NS045892/NS/NINDS NIH HHS/United States
GR  - U01 DK085532/DK/NIDDK NIH HHS/United States
GR  - KL2 RR025746/RR/NCRR NIH HHS/United States
GR  - KL2RR025746/RR/NCRR NIH HHS/United States
GR  - R01 DK091427/DK/NIDDK NIH HHS/United States
GR  - 5P30DK034987-27/DK/NIDDK NIH HHS/United States
GR  - U01 DK085507/DK/NIDDK NIH HHS/United States
GR  - K01 DK080181/DK/NIDDK NIH HHS/United States
GR  - P30CA06086/CA/NCI NIH HHS/United States
GR  - UL1RR025747/RR/NCRR NIH HHS/United States
GR  - UL1 RR025747/RR/NCRR NIH HHS/United States
GR  - R03 DK089126/DK/NIDDK NIH HHS/United States
GR  - P30NS045892/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Biomarkers)
RN  - 0 (CD24 Antigen)
RN  - 0 (Hyaluronan Receptors)
SB  - IM
MH  - Adult
MH  - Biomarkers/metabolism
MH  - CD24 Antigen/*metabolism
MH  - Epithelial Cells/*cytology
MH  - Female
MH  - Humans
MH  - Hyaluronan Receptors/*metabolism
MH  - Intestines/*cytology
MH  - Middle Aged
MH  - Stem Cells/*cytology/*metabolism
PMC - PMC3783577
MID - NIHMS468899
OTO - NOTNLM
OT  - Cell culture
OT  - Cell surface markers
OT  - Flow cytometry
OT  - Fluorescence-activated cell sorting
OT  - Tissue-specific stem cells
EDAT- 2013/04/05 06:00
MHDA- 2014/05/08 06:00
PMCR- 2014/09/01
CRDT- 2013/04/05 06:00
PHST- 2012/06/07 00:00 [received]
PHST- 2013/02/24 00:00 [accepted]
PHST- 2013/04/05 06:00 [entrez]
PHST- 2013/04/05 06:00 [pubmed]
PHST- 2014/05/08 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 10.1002/stem.1391 [doi]
PST - ppublish
SO  - Stem Cells. 2013 Sep;31(9):2024-30. doi: 10.1002/stem.1391.