PMID- 2369896
OWN - NLM
STAT- MEDLINE
DCOM- 19900822
LR  - 20230411
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 9
IP  - 8
DP  - 1990 Aug
TI  - Functionally distinct insulin receptors generated by tissue-specific alternative 
      splicing.
PG  - 2409-13
AB  - Cloning of the insulin receptor cDNA has earlier revealed the existence of two 
      alternative forms of the receptor differing by the presence or absence of 12 
      amino acids near the C-terminus of the receptor alpha-subunit. This insert has 
      been shown by others to be encoded by a discrete exon, and alternative splicing 
      of this exon leads to tissue-specific expression of two receptor isoforms. We 
      have studied the functional significance of the receptor isoforms and have 
      confirmed that they are generated by alternative splicing. When cDNAs encoding 
      the two forms of the insulin receptors are expressed in Rat 1 cells, the receptor 
      lacking the insert (HIR-A) has a significantly higher affinity for insulin than 
      the receptor with the insert (HIR-B). This difference in affinity is maintained 
      when insulin binding activity is assayed in solution using detergent solubilized, 
      partially purified receptors. These data, combined with the tissue specificity of 
      HIR-A and HIR-B expression, suggest that alternative splicing may result in the 
      modulation of insulin metabolism or responsiveness by different tissues.
FAU - Mosthaf, L
AU  - Mosthaf L
AD  - Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 
      90480.
FAU - Grako, K
AU  - Grako K
FAU - Dull, T J
AU  - Dull TJ
FAU - Coussens, L
AU  - Coussens L
FAU - Ullrich, A
AU  - Ullrich A
FAU - McClain, D A
AU  - McClain DA
LA  - eng
GR  - DK 33651/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Macromolecular Substances)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Gene Expression Regulation
MH  - Genomic Library
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - *RNA Splicing
MH  - Receptor, Insulin/*genetics/metabolism
MH  - Restriction Mapping
MH  - Transfection
PMC - PMC552265
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
PMCR- 1991/08/01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
PHST- 1991/08/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1990.tb07416.x [doi]
PST - ppublish
SO  - EMBO J. 1990 Aug;9(8):2409-13. doi: 10.1002/j.1460-2075.1990.tb07416.x.