PMID- 23744940
OWN - NLM
STAT- MEDLINE
DCOM- 20130612
LR  - 20220317
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Linking)
VI  - 340
IP  - 6137
DP  - 2013 Jun 7
TI  - Growing self-organizing mini-guts from a single intestinal stem cell: mechanism 
      and applications.
PG  - 1190-4
LID - 10.1126/science.1234852 [doi]
AB  - Recent examples have highlighted how stem cells have the capability to initiate 
      morphogenesis in vitro; that is, to generate complex structures in culture that 
      closely parallel their in vivo counterparts. Lgr5, the receptor for the 
      Wnt-agonistic R-spondins, marks stem cells in multiple adult organs of mice and 
      humans. In R-spondin-based three-dimensional cultures, these Lgr5 stem cells can 
      grow into ever-expanding epithelial organoids that retain their original organ 
      identity. Single Lgr5 stem cells derived from the intestine can be cultured to 
      build epithelial structures that retain hallmarks of the in vivo epithelium. 
      Here, we review the mechanisms that support this notable example of 
      self-organization and discuss applications of this technology for stem cell 
      research, disease modeling (e.g., for colorectal cancer and cystic fibrosis), and 
      regenerative medicine.
FAU - Sato, Toshiro
AU  - Sato T
AD  - Department of Gastroenterology, Keio University School of Medicine, Tokyo, Japan. 
      t.sato@a7.keio.jp
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Ephrin-B1)
RN  - 0 (Lgr5 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Wnt Proteins)
SB  - IM
MH  - Adult Stem Cells/*physiology
MH  - Animals
MH  - Cell Count
MH  - *Cell Culture Techniques
MH  - Disease Models, Animal
MH  - Ephrin-B1/metabolism
MH  - Humans
MH  - Intestinal Mucosa/physiology
MH  - Intestine, Small/*growth & development
MH  - Mice
MH  - *Morphogenesis
MH  - Receptors, G-Protein-Coupled/genetics
MH  - *Regenerative Medicine
MH  - Stem Cell Niche
MH  - Wnt Proteins/metabolism
EDAT- 2013/06/08 06:00
MHDA- 2013/06/13 06:00
CRDT- 2013/06/08 06:00
PHST- 2013/06/08 06:00 [entrez]
PHST- 2013/06/08 06:00 [pubmed]
PHST- 2013/06/13 06:00 [medline]
AID - 340/6137/1190 [pii]
AID - 10.1126/science.1234852 [doi]
PST - ppublish
SO  - Science. 2013 Jun 7;340(6137):1190-4. doi: 10.1126/science.1234852.