PMID- 23764754
OWN - NLM
STAT- MEDLINE
DCOM- 20140122
LR  - 20211021
IS  - 1460-2180 (Electronic)
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 34
IP  - 11
DP  - 2013 Nov
TI  - IMP1 promotes tumor growth, dissemination and a tumor-initiating cell phenotype 
      in colorectal cancer cell xenografts.
PG  - 2647-54
LID - 10.1093/carcin/bgt217 [doi]
AB  - Igf2 mRNA binding protein 1 (IMP1, CRD-BP, ZBP-1) is a messenger RNA binding 
      protein that we have shown previously to regulate colorectal cancer (CRC) cell 
      growth in vitro. Furthermore, increased IMP1 expression correlates with enhanced 
      metastasis and poor prognosis in CRC patients. In the current study, we sought to 
      elucidate IMP1-mediated functions in CRC pathogenesis in vivo. Using CRC cell 
      xenografts, we demonstrate that IMP1 overexpression promotes xenograft tumor 
      growth and dissemination into the blood. Furthermore, intestine-specific 
      knockdown of Imp1 dramatically reduces tumor number in the Apc (Min/+) mouse 
      model of intestinal tumorigenesis. In addition, IMP1 knockdown xenografts exhibit 
      a reduced number of tumor cells entering the circulation, suggesting that IMP1 
      may directly modulate this early metastatic event. We further demonstrate that 
      IMP1 overexpression decreases E-cadherin expression, promotes survival of single 
      tumor cell-derived colonospheres and promotes enrichment and maintenance of a 
      population of CD24+CD44+ cells, signifying that IMP1 overexpressing cells display 
      evidence of loss of epithelial identity and enhancement of a tumor-initiating 
      cell phenotype. Taken together, these findings implicate IMP1 as a modulator of 
      tumor growth and provide evidence for a novel role of IMP1 in early events in CRC 
      metastasis.
FAU - Hamilton, Kathryn E
AU  - Hamilton KE
AD  - Division of Gastroenterology, Department of Medicine, University of Pennsylvania, 
      Philadelphia, PA 19104, USA.
FAU - Noubissi, Felicite K
AU  - Noubissi FK
FAU - Katti, Prateek S
AU  - Katti PS
FAU - Hahn, Christopher M
AU  - Hahn CM
FAU - Davey, Sonya R
AU  - Davey SR
FAU - Lundsmith, Emma T
AU  - Lundsmith ET
FAU - Klein-Szanto, Andres J
AU  - Klein-Szanto AJ
FAU - Rhim, Andrew D
AU  - Rhim AD
FAU - Spiegelman, Vladimir S
AU  - Spiegelman VS
FAU - Rustgi, Anil K
AU  - Rustgi AK
LA  - eng
GR  - F32 DK093207-01/DK/NIDDK NIH HHS/United States
GR  - P30 DK050306/DK/NIDDK NIH HHS/United States
GR  - CA153102/CA/NCI NIH HHS/United States
GR  - T32 DK007066/DK/NIDDK NIH HHS/United States
GR  - P30 CA014520/CA/NCI NIH HHS/United States
GR  - AR063361/AR/NIAMS NIH HHS/United States
GR  - K08 DK088945/DK/NIDDK NIH HHS/United States
GR  - R01 DK056645-12/DK/NIDDK NIH HHS/United States
GR  - P30 CA016520/CA/NCI NIH HHS/United States
GR  - U01 DK085551/DK/NIDDK NIH HHS/United States
GR  - R01 AR063361/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130612
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Adenomatous Polyposis Coli Protein)
RN  - 0 (IMP1 protein, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Adenomatous Polyposis Coli Protein/*physiology
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Western
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cell Transformation, Neoplastic/metabolism/*pathology
MH  - Colorectal Neoplasms/metabolism/*pathology
MH  - Embryo, Mammalian/metabolism/pathology
MH  - Epithelial-Mesenchymal Transition
MH  - Fibroblasts/metabolism/pathology
MH  - Heterografts
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Integrases/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/*pathology
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Metastasis
MH  - Neoplastic Cells, Circulating/metabolism/pathology
MH  - Neoplastic Stem Cells/metabolism/*pathology
MH  - Phenotype
MH  - RNA-Binding Proteins/*physiology
MH  - Tumor Cells, Cultured
PMC - PMC3810836
EDAT- 2013/06/15 06:00
MHDA- 2014/01/23 06:00
PMCR- 2014/11/01
CRDT- 2013/06/15 06:00
PHST- 2013/06/15 06:00 [entrez]
PHST- 2013/06/15 06:00 [pubmed]
PHST- 2014/01/23 06:00 [medline]
PHST- 2014/11/01 00:00 [pmc-release]
AID - bgt217 [pii]
AID - 10.1093/carcin/bgt217 [doi]
PST - ppublish
SO  - Carcinogenesis. 2013 Nov;34(11):2647-54. doi: 10.1093/carcin/bgt217. Epub 2013 
      Jun 12.