PMID- 23785055 OWN - NLM STAT- MEDLINE DCOM- 20130925 LR - 20231213 IS - 1477-9129 (Electronic) IS - 0950-1991 (Print) IS - 0950-1991 (Linking) VI - 140 IP - 15 DP - 2013 Aug TI - TIE-DYE: a combinatorial marking system to visualize and genetically manipulate clones during development in Drosophila melanogaster. PG - 3275-84 LID - 10.1242/dev.096057 [doi] AB - Two types of information are particularly valuable in understanding the development of a tissue or an organ from a small population of founder cells. First, it is useful to know the composition of the final structure in terms the contribution of individual founder cells. Second, it is important to understand cell-cell interactions. To facilitate the study of both of these aspects of organ development at a tissue-wide level, we have developed a method, TIE-DYE, that allows simultaneous lineage tracing of multiple cell populations as well as the genetic manipulation of a subset of these populations. Seven uniquely marked categories of cells are produced by site-directed recombination of three independent cassettes. We have used the TIE-DYE method to estimate the number of founder cells that give rise to the wing-imaginal disc during normal development and following compensatory growth caused by X-ray irradiation of the founder cells. We also show that four out of the seven types of marked clones can be genetically manipulated by gene overexpression or RNAi knockdown, allowing an assessment of the consequences of these manipulations on the entire wing disc. We demonstrate the utility of this system in studying the consequences of alterations in growth, patterning and cell-cell affinity. FAU - Worley, Melanie I AU - Worley MI AD - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3200, USA. FAU - Setiawan, Linda AU - Setiawan L FAU - Hariharan, Iswar K AU - Hariharan IK LA - eng GR - R01 GM061672/GM/NIGMS NIH HHS/United States GR - R01 GM085576/GM/NIGMS NIH HHS/United States GR - R01 GM61672/GM/NIGMS NIH HHS/United States GR - R01 GM85576/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20130619 PL - England TA - Development JT - Development (Cambridge, England) JID - 8701744 RN - 0 (Drosophila Proteins) RN - 0 (GAL4 protein, Drosophila) RN - 0 (Luminescent Proteins) RN - 0 (Transcription Factors) RN - 147336-22-9 (Green Fluorescent Proteins) SB - IM MH - Animals MH - Animals, Genetically Modified MH - Body Patterning/genetics MH - Cell Lineage/*genetics MH - Clone Cells/cytology/metabolism MH - Drosophila Proteins/genetics MH - Drosophila melanogaster/cytology/*genetics/*growth & development MH - Female MH - Gene Expression Regulation, Developmental MH - *Genetic Techniques MH - Green Fluorescent Proteins/genetics MH - Imaginal Discs/cytology/growth & development MH - Luminescent Proteins/genetics MH - Male MH - Transcription Factors/genetics MH - Wings, Animal/cytology/growth & development MH - Red Fluorescent Protein PMC - PMC3931737 OTO - NOTNLM OT - Drosophila OT - Imaginal disc OT - Multicolor lineage technique EDAT- 2013/06/21 06:00 MHDA- 2013/09/26 06:00 PMCR- 2014/08/01 CRDT- 2013/06/21 06:00 PHST- 2013/06/21 06:00 [entrez] PHST- 2013/06/21 06:00 [pubmed] PHST- 2013/09/26 06:00 [medline] PHST- 2014/08/01 00:00 [pmc-release] AID - dev.096057 [pii] AID - 10.1242/dev.096057 [doi] PST - ppublish SO - Development. 2013 Aug;140(15):3275-84. doi: 10.1242/dev.096057. Epub 2013 Jun 19.