PMID- 24175801
OWN - NLM
STAT- MEDLINE
DCOM- 20141231
LR  - 20211203
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 14
IP  - 9
DP  - 2013
TI  - Effects of the hippo signaling pathway in human gastric cancer.
PG  - 5199-205
AB  - BACKGROUND/AIM: The Hippo signaling pathway is a newly discovered and conserved 
      signaling cascade, which regulates organ size control by governing cell 
      proliferation and apoptosis. This study aimed to investigate its effects in human 
      gastric cancer. METHODS: Tumor tissues (n=60), adjacent non-tumor tissues (n=60) 
      and normal tissues (n=60) were obtained from the same patients with primary 
      gastric cancer (GC). In addition, 70 samples of chronic atrophic gastritis (CAG) 
      tissues were obtained from patients with intestinal metaplasia (IM) by endoscopic 
      biopsy. Hippo signaling molecules, including Mst1, Lats1, YAP1, TAZ, TEAD1, Oct4 
      and CDX2, were determined by quantitative polymerase chain reaction (qPCR). 
      Protein expression of Mst1, Lats1, YAP1, TEAD1 and CDX2 was assessed by 
      immunohistochemistry and Western blotting. RESULTS: Mst1, Lats1 and Oct4 mRNA 
      expression showed an increasing tendency from GC tissues to normal gastric 
      tissues, while the mRNA expression of YAP1, TAZ and TEAD1 was up-regulated (all 
      P<0.01). Mst1 and Lats1 protein expression presented a similar trend with their 
      mRNA expression. In addition, YAP1 and TEAD1 protein expression in GC was 
      significantly higher than in the other groups (all P<0.01). CDX2 mRNA and protein 
      expression in the CAG group were higher than in the other groups (all P<0.01). In 
      GC, mRNA expression of Mst1, Lats1, Oct4, YAP1, TAZ, TEAD1 and CDX2 had a close 
      correlation with lymphatic metastasis and tumor TNM stage (all P<0.01). 
      Furthermore, protein expression of Mst1, Lats1 ,YAP1, TAZ, TEAD1 and CDX2 had a 
      close correlation between each other (P<0.05). CONCLUSION: The Hippo signaling 
      pathway is involved in the development, progression and metastasis of human 
      gastric cancer. Therefore, manipulation of Hippo signaling molecules may be a 
      potential therapeutic strategy for gastric cancer.
FAU - Zhou, Guang-Xi
AU  - Zhou GX
AD  - Department of Gastroenterology, The Affiliated Hospital of Qingdao University 
      Medical College, Qingdao, China E-mail : rose201001@126.com.
FAU - Li, Xiao-Yu
AU  - Li XY
FAU - Zhang, Qi
AU  - Zhang Q
FAU - Zhao, Kun
AU  - Zhao K
FAU - Zhang, Cui-Ping
AU  - Zhang CP
FAU - Xue, Chang-Hu
AU  - Xue CH
FAU - Yang, Kun
AU  - Yang K
FAU - Tian, Zi-Bin
AU  - Tian ZB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CDX2 Transcription Factor)
RN  - 0 (CDX2 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (TEA Domain Transcription Factors)
RN  - 0 (TEAD1 protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
RN  - 0 (macrophage stimulating protein)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (TAFAZZIN protein, human)
RN  - EC 2.7.1.- (LATS1 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Acyltransferases
MH  - Adaptor Proteins, Signal Transducing/genetics/metabolism
MH  - Apoptosis/genetics
MH  - Blotting, Western
MH  - CDX2 Transcription Factor
MH  - Carcinoma/*genetics/metabolism/pathology
MH  - Case-Control Studies
MH  - Cell Proliferation/genetics
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Gastritis, Atrophic/genetics/metabolism/pathology
MH  - *Gene Expression Regulation, Neoplastic
MH  - Hepatocyte Growth Factor/genetics/metabolism
MH  - Hippo Signaling Pathway
MH  - Homeodomain Proteins/genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Metaplasia/genetics/metabolism/pathology
MH  - Middle Aged
MH  - Nuclear Proteins/genetics/metabolism
MH  - Octamer Transcription Factor-3/genetics/metabolism
MH  - Phosphoproteins/genetics/metabolism
MH  - Precancerous Conditions/genetics/metabolism/pathology
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins/genetics/metabolism
MH  - RNA, Messenger/*genetics
MH  - Signal Transduction/*genetics/physiology
MH  - Stomach Neoplasms/*genetics/metabolism/pathology
MH  - TEA Domain Transcription Factors
MH  - Transcription Factors/genetics/metabolism
MH  - Up-Regulation
MH  - YAP-Signaling Proteins
EDAT- 2013/11/02 06:00
MHDA- 2015/01/01 06:00
CRDT- 2013/11/02 06:00
PHST- 2013/11/02 06:00 [entrez]
PHST- 2013/11/02 06:00 [pubmed]
PHST- 2015/01/01 06:00 [medline]
AID - 10.7314/apjcp.2013.14.9.5199 [doi]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2013;14(9):5199-205. doi: 10.7314/apjcp.2013.14.9.5199.