PMID- 24561995
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20211021
IS  - 1546-1726 (Electronic)
IS  - 1097-6256 (Linking)
VI  - 17
IP  - 4
DP  - 2014 Apr
TI  - Making Drosophila lineage-restricted drivers via patterned recombination in 
      neuroblasts.
PG  - 631-7
LID - 10.1038/nn.3654 [doi]
AB  - The Drosophila cerebrum originates from about 100 neuroblasts per hemisphere, 
      with each neuroblast producing a characteristic set of neurons. Neurons from a 
      neuroblast are often so diverse that many neuron types remain unexplored. We 
      developed new genetic tools that target neuroblasts and their diverse 
      descendants, increasing our ability to study fly brain structure and development. 
      Common enhancer-based drivers label neurons on the basis of terminal identities 
      rather than origins, which provides limited labeling in the heterogeneous 
      neuronal lineages. We successfully converted conventional drivers that are 
      temporarily expressed in neuroblasts, into drivers expressed in all subsequent 
      neuroblast progeny. One technique involves immortalizing GAL4 expression in 
      neuroblasts and their descendants. Another depends on loss of the GAL4 repressor, 
      GAL80, from neuroblasts during early neurogenesis. Furthermore, we expanded the 
      diversity of MARCM-based reagents and established another site-specific mitotic 
      recombination system. Our transgenic tools can be combined to map individual 
      neurons in specific lineages of various genotypes.
FAU - Awasaki, Takeshi
AU  - Awasaki T
AD  - 1] Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, 
      Virginia, USA. [2] Kyorin University School of Medicine, Mitaka, Tokyo, Japan. 
      [3].
FAU - Kao, Chih-Fei
AU  - Kao CF
AD  - 1] Department of Neurobiology, University of Massachusetts, Worcester, 
      Massachusetts, USA. [2] [3].
FAU - Lee, Ying-Jou
AU  - Lee YJ
AD  - 1] Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, 
      Virginia, USA. [2].
FAU - Yang, Ching-Po
AU  - Yang CP
AD  - 1] Department of Neurobiology, University of Massachusetts, Worcester, 
      Massachusetts, USA. [2].
FAU - Huang, Yaling
AU  - Huang Y
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - Pfeiffer, Barret D
AU  - Pfeiffer BD
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - Luan, Haojiang
AU  - Luan H
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - Jing, Xiaotang
AU  - Jing X
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - Huang, Yu-Fen
AU  - Huang YF
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - He, Yisheng
AU  - He Y
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - Schroeder, Mark David
AU  - Schroeder MD
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - Kuzin, Alexander
AU  - Kuzin A
AD  - Neural Cell-Fate Determinants Section, National Institute of Neurological 
      Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
FAU - Brody, Thomas
AU  - Brody T
AD  - Neural Cell-Fate Determinants Section, National Institute of Neurological 
      Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
FAU - Zugates, Christopher T
AU  - Zugates CT
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, 
      USA.
FAU - Odenwald, Ward F
AU  - Odenwald WF
AD  - Neural Cell-Fate Determinants Section, National Institute of Neurological 
      Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
FAU - Lee, Tzumin
AU  - Lee T
AD  - 1] Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, 
      Virginia, USA. [2] Department of Neurobiology, University of Massachusetts, 
      Worcester, Massachusetts, USA.
LA  - eng
GR  - R01 MH080739/MH/NIMH NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Technical Report
DEP - 20140223
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Drosophila Proteins)
RN  - 0 (N protein, Drosophila)
RN  - 0 (Receptors, Notch)
SB  - IM
MH  - Animals
MH  - *Cell Lineage/physiology
MH  - Cerebrum/*cytology/physiology
MH  - Drosophila/*cytology/physiology
MH  - *Drosophila Proteins/biosynthesis/genetics
MH  - *Genetic Techniques
MH  - Neural Stem Cells/*cytology/metabolism/physiology
MH  - Receptors, Notch/biosynthesis/genetics
MH  - Recombination, Genetic
MH  - Transgenes
EDAT- 2014/02/25 06:00
MHDA- 2014/06/03 06:00
CRDT- 2014/02/25 06:00
PHST- 2013/07/31 00:00 [received]
PHST- 2014/01/22 00:00 [accepted]
PHST- 2014/02/25 06:00 [entrez]
PHST- 2014/02/25 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
AID - nn.3654 [pii]
AID - 10.1038/nn.3654 [doi]
PST - ppublish
SO  - Nat Neurosci. 2014 Apr;17(4):631-7. doi: 10.1038/nn.3654. Epub 2014 Feb 23.