PMID- 24651727
OWN - NLM
STAT- MEDLINE
DCOM- 20151020
LR  - 20220317
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 4
DP  - 2014 Mar 21
TI  - Immune privilege of the CNS is not the consequence of limited antigen sampling.
PG  - 4422
LID - 10.1038/srep04422 [doi]
LID - 4422
AB  - Central nervous system (CNS) immune privilege is complex, and it is still not 
      understood how CNS antigens are sampled by the peripheral immune system under 
      steady state conditions. To compare antigen sampling from immune-privileged or 
      nonprivileged tissues, we created transgenic mice with oligodendrocyte or gut 
      epithelial cell expression of an EGFP-tagged fusion protein containing ovalbumin 
      (OVA) antigenic peptides and tested peripheral anti-OVA peptide-specific sentinel 
      OT-I and OT-II T cell activation. We report that oligodendrocyte or gut antigens 
      are sampled similarly, as determined by comparable levels of OT-I T cell 
      activation. However, activated T cells do not access the CNS under steady state 
      conditions. These data show that afferent immunity is normally intact as there is 
      no barrier at the antigen sampling level, but that efferent immunity is 
      restricted. To understand how this one-sided surveillance contributes to CNS 
      immune privilege will help us define mechanisms of CNS autoimmune disease 
      initiation.
FAU - Harris, Melissa G
AU  - Harris MG
AD  - 1] Department of Pathology and Laboratory Medicine, University of 
      Wisconsin-Madison, Madison, WI [2] Neuroscience Training Program, University of 
      Wisconsin-Madison, Madison, WI.
FAU - Hulseberg, Paul
AU  - Hulseberg P
AD  - 1] Department of Pathology and Laboratory Medicine, University of 
      Wisconsin-Madison, Madison, WI [2] Cellular and Molecular Pathology Graduate 
      Program, University of Wisconsin-Madison, Madison, WI.
FAU - Ling, Changying
AU  - Ling C
AD  - 1] Department of Pathology and Laboratory Medicine, University of 
      Wisconsin-Madison, Madison, WI [2] Department of Surgery, School of Medicine and 
      Public Health, University of Wisconsin-Madison, Madison, WI.
FAU - Karman, Jozsef
AU  - Karman J
AD  - 1] Department of Pathology and Laboratory Medicine, University of 
      Wisconsin-Madison, Madison, WI [2] Genzyme Corporation, Cambridge, MA.
FAU - Clarkson, Benjamin D
AU  - Clarkson BD
AD  - 1] Department of Pathology and Laboratory Medicine, University of 
      Wisconsin-Madison, Madison, WI [2] Cellular and Molecular Pathology Graduate 
      Program, University of Wisconsin-Madison, Madison, WI.
FAU - Harding, Jeffrey S
AU  - Harding JS
AD  - 1] Department of Pathology and Laboratory Medicine, University of 
      Wisconsin-Madison, Madison, WI [2] Cellular and Molecular Pathology Graduate 
      Program, University of Wisconsin-Madison, Madison, WI.
FAU - Zhang, Mengxue
AU  - Zhang M
AD  - Department of Pathology, Peking University, Beijing, China.
FAU - Sandor, Adam
AU  - Sandor A
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, WI.
FAU - Christensen, Kelsey
AU  - Christensen K
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, WI.
FAU - Nagy, Andras
AU  - Nagy A
AD  - Mount Sinai Hospital, Toronto, Ontario.
FAU - Sandor, Matyas
AU  - Sandor M
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, WI.
FAU - Fabry, Zsuzsanna
AU  - Fabry Z
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, WI.
LA  - eng
GR  - T32 GM081061/GM/NIGMS NIH HHS/United States
GR  - R01-AI048087/AI/NIAID NIH HHS/United States
GR  - R01-NS37570/NS/NINDS NIH HHS/United States
GR  - T32 GM007507/GM/NIGMS NIH HHS/United States
GR  - R01 AI048087/AI/NIAID NIH HHS/United States
GR  - R01 NS037570/NS/NINDS NIH HHS/United States
GR  - T32-GM007507/GM/NIGMS NIH HHS/United States
GR  - R01 NS076946/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140321
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antigens)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Adaptive Immunity
MH  - Animals
MH  - Antigens/chemistry/*immunology
MH  - CD4-Positive T-Lymphocytes/cytology/immunology
MH  - CD8-Positive T-Lymphocytes/cytology/immunology
MH  - Central Nervous System/*immunology/metabolism
MH  - Epithelial Cells/cytology/*immunology
MH  - Female
MH  - Gene Expression
MH  - Green Fluorescent Proteins/genetics/immunology
MH  - *Immunity, Innate
MH  - Intestinal Mucosa/cytology/*immunology
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Oligodendroglia/cytology/*immunology
MH  - Ovalbumin/genetics/immunology
MH  - Recombinant Fusion Proteins/genetics/immunology
PMC - PMC3961746
EDAT- 2014/03/22 06:00
MHDA- 2015/10/21 06:00
PMCR- 2014/03/21
CRDT- 2014/03/22 06:00
PHST- 2014/01/09 00:00 [received]
PHST- 2014/02/21 00:00 [accepted]
PHST- 2014/03/22 06:00 [entrez]
PHST- 2014/03/22 06:00 [pubmed]
PHST- 2015/10/21 06:00 [medline]
PHST- 2014/03/21 00:00 [pmc-release]
AID - srep04422 [pii]
AID - 10.1038/srep04422 [doi]
PST - epublish
SO  - Sci Rep. 2014 Mar 21;4:4422. doi: 10.1038/srep04422.