PMID- 2499043 OWN - NLM STAT- MEDLINE DCOM- 19890623 LR - 20190618 IS - 0036-8075 (Print) IS - 0036-8075 (Linking) VI - 244 IP - 4906 DP - 1989 May 19 TI - Golf: an olfactory neuron specific-G protein involved in odorant signal transduction. PG - 790-5 AB - Biochemical and electrophysiological studies suggest that odorants induce responses in olfactory sensory neurons via an adenylate cyclase cascade mediated by a G protein. An olfactory-specific guanosine triphosphate (GTP)-binding protein alpha subunit has now been characterized and evidence is presented suggesting that this G protein, termed Golf, mediates olfaction. Messenger RNA that encodes Golf alpha is expressed in olfactory neuroephithelium but not in six other tissues tested. Moreover, within the olfactory epithelium, Golf alpha appears to be expressed only by the sensory neurons. Specific antisera were used to localize Golf alpha protein to the sensory apparatus of the receptor neurons. Golf alpha shares extensive amino acid identity (88 percent) with the stimulatory G protein, Gs alpha. The expression of Golf alpha in S49 cyc- kin- cells, a line deficient in endogenous stimulatory G proteins, demonstrates its capacity to stimulate adenylate cyclase in a heterologous system. FAU - Jones, D T AU - Jones DT AD - Howard Hughes Medical Institute, Department of Molecular Biology and Genetic Johns Hopkins School of Medicine, Baltimore, MD 21205. FAU - Reed, R R AU - Reed RR LA - eng SI - GENBANK/M26718 PT - Comparative Study PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Science JT - Science (New York, N.Y.) JID - 0404511 RN - 0 (RNA, Messenger) RN - EC 3.6.1.- (GTP-Binding Proteins) RN - EC 4.6.1.1 (Adenylyl Cyclases) SB - IM MH - Adenylyl Cyclases/metabolism MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Cloning, Molecular MH - GTP-Binding Proteins/analysis/genetics/*physiology MH - Gene Expression Regulation MH - Immunoblotting MH - Immunohistochemistry MH - Molecular Sequence Data MH - Neurons, Afferent/analysis/*physiology MH - *Odorants MH - Olfactory Bulb/physiology MH - Olfactory Mucosa/analysis/*innervation MH - RNA, Messenger/analysis/genetics MH - Rats MH - Sequence Homology, Nucleic Acid MH - *Signal Transduction MH - Tissue Distribution MH - Transfection EDAT- 1989/05/19 00:00 MHDA- 1989/05/19 00:01 CRDT- 1989/05/19 00:00 PHST- 1989/05/19 00:00 [pubmed] PHST- 1989/05/19 00:01 [medline] PHST- 1989/05/19 00:00 [entrez] AID - 10.1126/science.2499043 [doi] PST - ppublish SO - Science. 1989 May 19;244(4906):790-5. doi: 10.1126/science.2499043.