PMID- 25119793
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR  - 20231110
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Print)
IS  - 1936-0851 (Linking)
VI  - 8
IP  - 10
DP  - 2014 Oct 28
TI  - Bromelain surface modification increases the diffusion of silica nanoparticles in 
      the tumor extracellular matrix.
PG  - 9874-83
LID - 10.1021/nn502807n [doi]
AB  - Tumor extracellular matrix (ECM) represents a major obstacle to the diffusion of 
      therapeutics and drug delivery systems in cancer parenchyma. This biological 
      barrier limits the efficacy of promising therapeutic approaches including the 
      delivery of siRNA or agents intended for thermoablation. After extravasation due 
      to the enhanced penetration and retention effect of tumor vasculature, typical 
      nanotherapeutics are unable to reach the nonvascularized and anoxic regions deep 
      within cancer parenchyma. Here, we developed a simple method to provide 
      mesoporous silica nanoparticles (MSN) with a proteolytic surface. To this extent, 
      we chose to conjugate MSN to Bromelain (Br-MSN), a crude enzymatic complex, 
      purified from pineapple stems, that belongs to the peptidase papain family. This 
      surface modification increased particle uptake in endothelial, macrophage, and 
      cancer cell lines with minimal impact on cellular viability. Most importantly 
      Br-MSN showed an increased ability to digest and diffuse in tumor ECM in vitro 
      and in vivo.
FAU - Parodi, Alessandro
AU  - Parodi A
AD  - Department of Nanomedicine, Houston Methodist Research Institute , Houston, Texas 
      77030, United States.
FAU - Haddix, Seth G
AU  - Haddix SG
FAU - Taghipour, Nima
AU  - Taghipour N
FAU - Scaria, Shilpa
AU  - Scaria S
FAU - Taraballi, Francesca
AU  - Taraballi F
FAU - Cevenini, Armando
AU  - Cevenini A
FAU - Yazdi, Iman K
AU  - Yazdi IK
FAU - Corbo, Claudia
AU  - Corbo C
FAU - Palomba, Roberto
AU  - Palomba R
FAU - Khaled, Sm Z
AU  - Khaled SZ
FAU - Martinez, Jonathan O
AU  - Martinez JO
FAU - Brown, Brandon S
AU  - Brown BS
FAU - Isenhart, Lucas
AU  - Isenhart L
FAU - Tasciotti, Ennio
AU  - Tasciotti E
LA  - eng
GR  - F31 CA154119/CA/NCI NIH HHS/United States
GR  - R21 CA173579/CA/NCI NIH HHS/United States
GR  - 1R21CA173579-01A1/CA/NCI NIH HHS/United States
GR  - 1F31CA154119/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140917
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 9001-00-7 (Bromelains)
SB  - IM
MH  - Bromelains/*chemistry
MH  - Diffusion
MH  - Extracellular Matrix/*chemistry
MH  - Microscopy, Electron, Transmission
MH  - *Nanoparticles
MH  - Neoplasms/blood supply/*chemistry/pathology
MH  - Silicon Dioxide/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Surface Properties
PMC - PMC4212787
OTO - NOTNLM
OT  - Bromelain
OT  - cancer
OT  - cellular uptake
OT  - extracellular matrix digestion
OT  - mesoporous silica nanoparticle modification
EDAT- 2014/08/15 06:00
MHDA- 2015/10/01 06:00
PMCR- 2015/08/13
CRDT- 2014/08/15 06:00
PHST- 2014/08/15 06:00 [entrez]
PHST- 2014/08/15 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
PHST- 2015/08/13 00:00 [pmc-release]
AID - 10.1021/nn502807n [doi]
PST - ppublish
SO  - ACS Nano. 2014 Oct 28;8(10):9874-83. doi: 10.1021/nn502807n. Epub 2014 Sep 17.