PMID- 25762175
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20240104
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 125
IP  - 17
DP  - 2015 Apr 23
TI  - Hematopoietic stem cells: concepts, definitions, and the new reality.
PG  - 2605-13
LID - 10.1182/blood-2014-12-570200 [doi]
AB  - Hematopoietic stem cell (HSC) research took hold in the 1950s with the 
      demonstration that intravenously injected bone marrow cells can rescue irradiated 
      mice from lethality by reestablishing blood cell production. Attempts to quantify 
      the cells responsible led to the discovery of serially transplantable, 
      donor-derived, macroscopic, multilineage colonies detectable on the spleen 
      surface 1 to 2 weeks posttransplant. The concept of self-renewing multipotent 
      HSCs was born, but accompanied by perplexing evidence of great variability in the 
      outcomes of HSC self-renewal divisions. The next 60 years saw an explosion in the 
      development and use of more refined tools for assessing the behavior of 
      prospectively purified subsets of hematopoietic cells with blood cell-producing 
      capacity. These developments have led to the formulation of increasingly complex 
      hierarchical models of hematopoiesis and a growing list of intrinsic and 
      extrinsic elements that regulate HSC cycling status, viability, self-renewal, and 
      lineage outputs. More recent examination of these properties in individual, 
      highly purified HSCs and analyses of their perpetuation in clonally generated 
      progeny HSCs have now provided definitive evidence of linearly transmitted 
      heterogeneity in HSC states. These results anticipate the need and use of 
      emerging new technologies to establish models that will accommodate such 
      pluralistic features of HSCs and their control mechanisms.
CI  - (c) 2015 by The American Society of Hematology.
FAU - Eaves, Connie J
AU  - Eaves CJ
AUID- ORCID: 0000-0002-3452-1926
AD  - Terry Fox Laboratory, British Columbia Cancer Agency Vancouver, BC, Canada; and 
      Department of Medical Genetics, University of British Columbia, Vancouver, BC, 
      Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20150311
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
CIN - Boulais
CIN - Vo
MH  - Animals
MH  - Cell Culture Techniques/methods
MH  - Cell Separation/methods
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cell Transplantation/methods
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
PMC - PMC4440889
EDAT- 2015/03/13 06:00
MHDA- 2015/06/30 06:00
PMCR- 2015/04/23
CRDT- 2015/03/13 06:00
PHST- 2014/12/12 00:00 [received]
PHST- 2015/02/03 00:00 [accepted]
PHST- 2015/03/13 06:00 [entrez]
PHST- 2015/03/13 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2015/04/23 00:00 [pmc-release]
AID - S0006-4971(20)41227-3 [pii]
AID - 2014/570200 [pii]
AID - 10.1182/blood-2014-12-570200 [doi]
PST - ppublish
SO  - Blood. 2015 Apr 23;125(17):2605-13. doi: 10.1182/blood-2014-12-570200. Epub 2015 
      Mar 11.