PMID- 25811596
OWN - NLM
STAT- MEDLINE
DCOM- 20160229
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 3
DP  - 2015
TI  - Bactericidal activity of curcumin I is associated with damaging of bacterial 
      membrane.
PG  - e0121313
LID - 10.1371/journal.pone.0121313 [doi]
LID - e0121313
AB  - Curcumin, an important constituent of turmeric, is known for various biological 
      activities, primarily due to its antioxidant mechanism. The present study focused 
      on the antibacterial activity of curcumin I, a significant component of 
      commercial curcumin, against four genera of bacteria, including those that are 
      Gram-positive (Staphylococcus aureus and Enterococcus faecalis) and Gram-negative 
      (Escherichia coli and Pseudomonas aeruginosa). These represent prominent human 
      pathogens, particularly in hospital settings. Our study shows the strong 
      antibacterial potential of curcumin I against all the tested bacteria from 
      Gram-positive as well as Gram-negative groups. The integrity of the bacterial 
      membrane was checked using two differential permeabilization indicating 
      fluorescent probes, namely, propidium iodide and calcein. Both the membrane 
      permeabilization assays confirmed membrane leakage in Gram-negative and 
      Gram-positive bacteria on exposure to curcumin I. In addition, scanning electron 
      microscopy and fluorescence microscopy were employed to confirm the membrane 
      damages in bacterial cells on exposure to curcumin I. The present study confirms 
      the broad-spectrum antibacterial nature of curcumin I, and its membrane damaging 
      property. Findings from this study could provide impetus for further research on 
      curcumin I regarding its antibiotic potential against rapidly emerging bacterial 
      pathogens.
FAU - Tyagi, Poonam
AU  - Tyagi P
AD  - School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, 
      India.
FAU - Singh, Madhuri
AU  - Singh M
AD  - School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, 
      India.
FAU - Kumari, Himani
AU  - Kumari H
AD  - School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, 
      India.
FAU - Kumari, Anita
AU  - Kumari A
AD  - School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, 
      India.
FAU - Mukhopadhyay, Kasturi
AU  - Mukhopadhyay K
AD  - School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, 
      India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150326
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fluoresceins)
RN  - 36015-30-2 (Propidium)
RN  - IT942ZTH98 (Curcumin)
RN  - V0YM2B16TS (fluorexon)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacteria/*cytology/*drug effects/ultrastructure
MH  - Cell Membrane/*drug effects/ultrastructure
MH  - Cell Membrane Permeability/drug effects
MH  - Colony Count, Microbial
MH  - Curcumin/*pharmacology
MH  - Flow Cytometry
MH  - Fluoresceins/metabolism
MH  - Kinetics
MH  - Microbial Sensitivity Tests
MH  - Microbial Viability/drug effects
MH  - Microscopy, Fluorescence
MH  - Propidium/metabolism
MH  - Spectrometry, Fluorescence
PMC - PMC4374920
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/03/27 06:00
MHDA- 2016/03/02 06:00
PMCR- 2015/03/26
CRDT- 2015/03/27 06:00
PHST- 2014/07/28 00:00 [received]
PHST- 2015/01/30 00:00 [accepted]
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
PHST- 2015/03/26 00:00 [pmc-release]
AID - PONE-D-14-33747 [pii]
AID - 10.1371/journal.pone.0121313 [doi]
PST - epublish
SO  - PLoS One. 2015 Mar 26;10(3):e0121313. doi: 10.1371/journal.pone.0121313. 
      eCollection 2015.