PMID- 25865482
OWN - NLM
STAT- MEDLINE
DCOM- 20150706
LR  - 20190108
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 161
IP  - 3
DP  - 2015 Apr 23
TI  - Bending gradients: how the intestinal stem cell gets its home.
PG  - 569-580
LID - S0092-8674(15)00361-X [pii]
LID - 10.1016/j.cell.2015.03.041 [doi]
AB  - We address the mechanism by which adult intestinal stem cells (ISCs) become 
      localized to the base of each villus during embryonic development. We find that, 
      early in gut development, proliferating progenitors expressing ISC markers are 
      evenly distributed throughout the epithelium, in both the chick and mouse. 
      However, as the villi form, the putative stem cells become restricted to the base 
      of the villi. This shift in the localization is driven by mechanically influenced 
      reciprocal signaling between the epithelium and underlying mesenchyme. Buckling 
      forces physically distort the shape of the morphogenic field, causing local 
      maxima of epithelial signals, in particular Shh, at the tip of each villus. This 
      induces a suite of high-threshold response genes in the underlying mesenchyme to 
      form a signaling center called the "villus cluster." Villus cluster signals, 
      notably Bmp4, feed back on the overlying epithelium to ultimately restrict the 
      stem cells to the base of each villus.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Shyer, Amy E
AU  - Shyer AE
AD  - Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
FAU - Huycke, Tyler R
AU  - Huycke TR
AD  - Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
FAU - Lee, ChangHee
AU  - Lee C
AD  - Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
FAU - Mahadevan, L
AU  - Mahadevan L
AD  - School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 
      02138, USA; Department of Organismic and Evolutionary Biology, Harvard 
      University, Cambridge, MA 02138, USA; Department of Physics, Harvard University, 
      Cambridge, MA 02138, USA; Wyss Institute for Biologically Inspired Engineering, 
      Harvard University, Cambridge, MA 02138, USA; Kavli Institute for Nanobio Science 
      and Technology, Harvard University, Cambridge, MA 02138, USA; Department of 
      Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
FAU - Tabin, Clifford J
AU  - Tabin CJ
AD  - Department of Genetics, Harvard Medical School, Boston, MA 02115, USA. Electronic 
      address: tabin@genetics.med.harvard.edu.
LA  - eng
GR  - F32 DK103563/DK/NIDDK NIH HHS/United States
GR  - R01 HD047360/HD/NICHD NIH HHS/United States
GR  - HD047360/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150409
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Avian Proteins)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
CIN - Cell. 2015 Apr 23;161(3):431-432. doi: 10.1016/j.cell.2015.04.009. PMID: 25910201
MH  - Adult Stem Cells/*cytology/metabolism
MH  - Animals
MH  - Avian Proteins/analysis/metabolism
MH  - Biomechanical Phenomena
MH  - Chick Embryo
MH  - Hedgehog Proteins/metabolism
MH  - Intestine, Small/*cytology/embryology/metabolism
MH  - *Mechanotransduction, Cellular
MH  - Mice
MH  - Morphogenesis
MH  - Receptors, G-Protein-Coupled/analysis
MH  - Signal Transduction
PMC - PMC4409931
MID - NIHMS675970
EDAT- 2015/04/14 06:00
MHDA- 2015/07/07 06:00
PMCR- 2016/04/23
CRDT- 2015/04/14 06:00
PHST- 2014/06/17 00:00 [received]
PHST- 2014/10/28 00:00 [revised]
PHST- 2015/02/11 00:00 [accepted]
PHST- 2015/04/14 06:00 [entrez]
PHST- 2015/04/14 06:00 [pubmed]
PHST- 2015/07/07 06:00 [medline]
PHST- 2016/04/23 00:00 [pmc-release]
AID - S0092-8674(15)00361-X [pii]
AID - 10.1016/j.cell.2015.03.041 [doi]
PST - ppublish
SO  - Cell. 2015 Apr 23;161(3):569-580. doi: 10.1016/j.cell.2015.03.041. Epub 2015 Apr 
      9.