PMID- 25902518 OWN - NLM STAT- MEDLINE DCOM- 20150803 LR - 20190524 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 112 IP - 18 DP - 2015 May 5 TI - During Drosophila disc regeneration, JAK/STAT coordinates cell proliferation with Dilp8-mediated developmental delay. PG - E2327-36 LID - 10.1073/pnas.1423074112 [doi] AB - Regeneration of fragmented Drosophila imaginal discs occurs in an epimorphic manner involving local cell proliferation at the wound site. After disc fragmentation, cells at the wound site activate a restoration program through wound healing, regenerative cell proliferation, and repatterning of the tissue. However, the interplay of signaling cascades driving these early reprogramming steps is not well-understood. Here, we profiled the transcriptome of regenerating cells in the early phase within 24 h after wounding. We found that JAK/STAT signaling becomes activated at the wound site and promotes regenerative cell proliferation in cooperation with Wingless (Wg) signaling. In addition, we showed that the expression of Drosophila insulin-like peptide 8 (dilp8), which encodes a paracrine peptide to delay the onset of pupariation, is controlled by JAK/STAT signaling in early regenerating discs. Our findings suggest that JAK/STAT signaling plays a pivotal role in coordinating regenerative disc growth with organismal developmental timing. FAU - Katsuyama, Tomonori AU - Katsuyama T AD - Department of Biosystems Science and Engineering, Eidgenossische Technische Hochschule Zurich, 4058 Basel, Switzerland; FAU - Comoglio, Federico AU - Comoglio F AD - Department of Biosystems Science and Engineering, Eidgenossische Technische Hochschule Zurich, 4058 Basel, Switzerland; FAU - Seimiya, Makiko AU - Seimiya M AD - Department of Biosystems Science and Engineering, Eidgenossische Technische Hochschule Zurich, 4058 Basel, Switzerland; FAU - Cabuy, Erik AU - Cabuy E AD - Single Cell Genomics, Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland; and. FAU - Paro, Renato AU - Paro R AD - Department of Biosystems Science and Engineering, Eidgenossische Technische Hochschule Zurich, 4058 Basel, Switzerland; Faculty of Science, University of Basel, 4056 Basel, Switzerland renato.paro@bsse.ethz.ch. LA - eng SI - GEO/GSE54868 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150420 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Drosophila Proteins) RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (STAT Transcription Factors) RN - 0 (Transcription Factors) RN - 0 (insulin-like peptide 8, Drosophila) RN - EC 2.7.10.2 (Janus Kinases) RN - EC 2.7.10.2 (hop protein, Drosophila) SB - IM MH - Animals MH - Body Patterning MH - Cell Lineage MH - Cell Proliferation MH - Cluster Analysis MH - Drosophila/*physiology MH - Drosophila Proteins/*metabolism MH - Gene Expression Regulation MH - Imaginal Discs/*physiology MH - Intercellular Signaling Peptides and Proteins/*metabolism MH - Janus Kinases/metabolism MH - Oligonucleotide Array Sequence Analysis MH - Principal Component Analysis MH - Regeneration MH - STAT Transcription Factors/*metabolism MH - Signal Transduction MH - Transcription Factors/metabolism MH - Transcriptome MH - *Wound Healing PMC - PMC4426433 OTO - NOTNLM OT - Drosophila imaginal discs OT - JAK/STAT signaling OT - epimorphic regeneration COIS- The authors declare no conflict of interest. EDAT- 2015/04/23 06:00 MHDA- 2015/08/04 06:00 PMCR- 2015/04/20 CRDT- 2015/04/23 06:00 PHST- 2015/04/23 06:00 [entrez] PHST- 2015/04/23 06:00 [pubmed] PHST- 2015/08/04 06:00 [medline] PHST- 2015/04/20 00:00 [pmc-release] AID - 1423074112 [pii] AID - 201423074 [pii] AID - 10.1073/pnas.1423074112 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2015 May 5;112(18):E2327-36. doi: 10.1073/pnas.1423074112. Epub 2015 Apr 20.