PMID- 26510502
OWN - NLM
STAT- MEDLINE
DCOM- 20161101
LR  - 20181113
IS  - 1939-4586 (Electronic)
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 27
IP  - 1
DP  - 2016 Jan 1
TI  - The endocytic recycling compartment maintains cargo segregation acquired upon 
      exit from the sorting endosome.
PG  - 108-26
LID - 10.1091/mbc.E15-07-0514 [doi]
AB  - The endocytic recycling compartment (ERC) is a series of perinuclear tubular and 
      vesicular membranes that regulates recycling to the plasma membrane. Despite 
      evidence that cargo is sorted at the early/sorting endosome (SE), whether cargo 
      mixes downstream at the ERC or remains segregated is an unanswered question. Here 
      we use three-dimensional (3D) structured illumination microscopy and dual-channel 
      and 3D direct stochastic optical reconstruction microscopy (dSTORM) to obtain new 
      information about ERC morphology and cargo segregation. We show that cargo 
      internalized either via clathrin-mediated endocytosis (CME) or independently of 
      clathrin (CIE) remains segregated in the ERC, likely on distinct carriers. This 
      suggests that no further sorting occurs upon cargo exit from SE. Moreover, 3D 
      dSTORM data support a model in which some but not all ERC vesicles are tethered 
      by contiguous "membrane bridges." Furthermore, tubular recycling endosomes 
      preferentially traffic CIE cargo and may originate from SE membranes. These 
      findings support a significantly altered model for endocytic recycling in 
      mammalian cells in which sorting occurs in peripheral endosomes and segregation 
      is maintained at the ERC.
CI  - (c) 2016 Xie et al. This article is distributed by The American Society for Cell 
      Biology under license from the author(s). Two months after publication it is 
      available to the public under an Attribution-Noncommercial-Share Alike 3.0 
      Unported Creative Commons License 
      (http://creativecommons.org/licenses/by-nc-sa/3.0).
FAU - Xie, Shuwei
AU  - Xie S
AD  - Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett 
      Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198-5870.
FAU - Bahl, Kriti
AU  - Bahl K
AD  - Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett 
      Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198-5870.
FAU - Reinecke, James B
AU  - Reinecke JB
AD  - Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett 
      Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198-5870.
FAU - Hammond, Gerald R V
AU  - Hammond GR
AD  - Department of Cell Biology, University of Pittsburgh School of Medicine, 
      Pittsburgh, PA 15261.
FAU - Naslavsky, Naava
AU  - Naslavsky N
AD  - Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett 
      Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198-5870.
FAU - Caplan, Steve
AU  - Caplan S
AD  - Department of Biochemistry and Molecular Biology and the Fred and Pamela Buffett 
      Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198-5870 
      scaplan@unmc.edu.
LA  - eng
GR  - R01GM074876/GM/NIGMS NIH HHS/United States
GR  - R01GM087455/GM/NIGMS NIH HHS/United States
GR  - R01 GM087455/GM/NIGMS NIH HHS/United States
GR  - P30 GM106397/GM/NIGMS NIH HHS/United States
GR  - P30 CA036727/CA/NCI NIH HHS/United States
GR  - P30GM106397/GM/NIGMS NIH HHS/United States
GR  - P20 RR018759/RR/NCRR NIH HHS/United States
GR  - R01 GM074876/GM/NIGMS NIH HHS/United States
GR  - P30CA036727/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20151028
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Clathrin)
RN  - 0 (Transferrin)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport/metabolism
MH  - Cell Membrane/metabolism
MH  - Clathrin/metabolism
MH  - Endocytosis/physiology
MH  - Endosomes/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Microscopy/methods
MH  - Protein Transport/physiology
MH  - Transferrin/metabolism
MH  - Transport Vesicles/*metabolism
PMC - PMC4694750
EDAT- 2015/10/30 06:00
MHDA- 2016/11/02 06:00
PMCR- 2016/03/16
CRDT- 2015/10/30 06:00
PHST- 2015/07/20 00:00 [received]
PHST- 2015/10/23 00:00 [accepted]
PHST- 2015/10/30 06:00 [entrez]
PHST- 2015/10/30 06:00 [pubmed]
PHST- 2016/11/02 06:00 [medline]
PHST- 2016/03/16 00:00 [pmc-release]
AID - mbc.E15-07-0514 [pii]
AID - E15-07-0514 [pii]
AID - 10.1091/mbc.E15-07-0514 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2016 Jan 1;27(1):108-26. doi: 10.1091/mbc.E15-07-0514. Epub 2015 
      Oct 28.