PMID- 26550824
OWN - NLM
STAT- MEDLINE
DCOM- 20151224
LR  - 20191114
IS  - 1476-4687 (Electronic)
IS  - 0028-0836 (Linking)
VI  - 527
IP  - 7578
DP  - 2015 Nov 19
TI  - Decapentaplegic and growth control in the developing Drosophila wing.
PG  - 375-8
LID - 10.1038/nature15730 [doi]
AB  - As a central model for morphogen action during animal development, the bone 
      morphogenetic protein 2/4 (BMP2/4)-like ligand Decapentaplegic (Dpp) is proposed 
      to form a long-range signalling gradient that directs both growth and pattern 
      formation during Drosophila wing disc development. While the patterning role of 
      Dpp secreted from a stripe of cells along the anterior-posterior compartmental 
      boundary is well established, the mechanism by which a Dpp gradient directs 
      uniform cell proliferation remains controversial and poorly understood. Here, to 
      determine the precise spatiotemporal requirements for Dpp during wing disc 
      development, we use CRISPR-Cas9-mediated genome editing to generate a flippase 
      recognition target (FRT)-dependent conditional null allele. By genetically 
      removing Dpp from its endogenous stripe domain, we confirm the requirement of Dpp 
      for the activation of a downstream phospho-Mothers against dpp (p-Mad) gradient 
      and the regulation of the patterning targets spalt (sal), optomotor blind (omb; 
      also known as bifid) and brinker (brk). Surprisingly, however, third-instar wing 
      blade primordia devoid of compartmental dpp expression maintain relatively normal 
      rates of cell proliferation and exhibit only mild defects in growth. These 
      results indicate that during the latter half of larval development, the Dpp 
      morphogen gradient emanating from the anterior-posterior compartment boundary is 
      not directly required for wing disc growth.
FAU - Akiyama, Takuya
AU  - Akiyama T
AD  - Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
FAU - Gibson, Matthew C
AU  - Gibson MC
AD  - Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
AD  - Department of Anatomy and Cell Biology, The University of Kansas School of 
      Medicine, Kansas City, Kansas 66160, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151109
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MAD protein, Drosophila)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (brk protein, Drosophila)
RN  - 0 (dpp protein, Drosophila)
RN  - 0 (salm protein, Drosophila)
RN  - 146635-31-6 (bi protein, Drosophila)
SB  - IM
CIN - Nat Rev Mol Cell Biol. 2016 Jan;17(1):1. PMID: 26607170
CIN - Curr Biol. 2016 Mar 7;26(5):R209-12. PMID: 26954443
MH  - Animals
MH  - CRISPR-Cas Systems/genetics
MH  - Cell Proliferation
MH  - DNA-Binding Proteins/metabolism
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Drosophila melanogaster/genetics/*growth & development/*metabolism
MH  - Homeodomain Proteins/metabolism
MH  - Morphogenesis
MH  - Nerve Tissue Proteins/metabolism
MH  - Repressor Proteins/metabolism
MH  - T-Box Domain Proteins/metabolism
MH  - Transcription Factors/metabolism
MH  - Wings, Animal/*growth & development/*metabolism
EDAT- 2015/11/10 06:00
MHDA- 2015/12/25 06:00
CRDT- 2015/11/10 06:00
PHST- 2015/03/13 00:00 [received]
PHST- 2015/09/14 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2015/12/25 06:00 [medline]
AID - nature15730 [pii]
AID - 10.1038/nature15730 [doi]
PST - ppublish
SO  - Nature. 2015 Nov 19;527(7578):375-8. doi: 10.1038/nature15730. Epub 2015 Nov 9.