PMID- 2714648
OWN - NLM
STAT- MEDLINE
DCOM- 19890622
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 3
IP  - 2
DP  - 1989 Feb
TI  - Genetic dissection of Drosophila myofibril formation: effects of actin and myosin 
      heavy chain null alleles.
PG  - 131-40
AB  - We used null mutations of Drosophila actin and myosin genes to investigate two 
      aspects of myofibril assembly. First, we eliminated all actin or myosin in flight 
      muscles to evaluate contributions of thick and thin filaments to sarcomere 
      formation. Results demonstrate that thick and thin filament arrays can assemble 
      independently but that both are essential for sarcomeric order and periodicity. 
      Second, we examined how filament stoichiometry affects myofibril assembly. We 
      find that heterozygotes for actin (Act88F) or myosin heavy chain (Mhc36B) null 
      alleles have complex myofibrillar defects, whereas Mhc36B-/+; Act88F-/+ double 
      heterozygotes have nearly normal myofibrils. These results imply that most 
      defects observed in single heterozygotes are due to filament imbalances, not 
      deficits, and suggest that thick and thin filament interactions regulate 
      myofibrillar growth and alignment.
FAU - Beall, C J
AU  - Beall CJ
AD  - Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
FAU - Sepanski, M A
AU  - Sepanski MA
FAU - Fyrberg, E A
AU  - Fyrberg EA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Actins)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Actins/biosynthesis/*genetics/physiology
MH  - Alleles
MH  - Animals
MH  - Drosophila/*genetics/growth & development
MH  - Heterozygote
MH  - Mutation
MH  - Myofibrils/*ultrastructure
MH  - Myosins/biosynthesis/*genetics/physiology
MH  - Wings, Animal/anatomy & histology
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
AID - 10.1101/gad.3.2.131 [doi]
PST - ppublish
SO  - Genes Dev. 1989 Feb;3(2):131-40. doi: 10.1101/gad.3.2.131.