PMID- 2721960
OWN - NLM
STAT- MEDLINE
DCOM- 19890710
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 3
IP  - 4
DP  - 1989 Apr
TI  - A nucleoprotein complex mediates the integration of retroviral DNA.
PG  - 469-78
AB  - The integration of viral DNA into the host genome is an essential step in the 
      retrovirus life cycle. To understand this process better, we have examined the 
      native state of viral DNA in cells acutely infected by murine leukemia virus 
      (MLV), using both a physical assay for viral DNA and a functional assay for 
      integration activity (Brown et al. 1987). The viral DNA and integration activity 
      copurify during velocity sedimentation, gel filtration, and density equilibrium 
      centrifugation, indicating that viral DNA is in a large (approximately 160S) 
      nucleoprotein complex that includes all functions required for integration 
      activity in vitro. Analysis by immunoprecipitation shows that the viral capsid 
      protein is part of the active nucleoprotein complex, but recognition of the 
      complex by only a subset of anti-capsid sera implies that the protein is 
      constrained conformationally. The viral DNA within this structure is accessible 
      to nucleases; the effects of nucleases on the integrity of the complex suggest 
      that the integration-competent particle is derived from and similar to the core 
      of extracellular virions.
FAU - Bowerman, B
AU  - Bowerman B
AD  - Department of Biochemistry and Biophysics; University of California, San 
      Francisco 94143.
FAU - Brown, P O
AU  - Brown PO
FAU - Bishop, J M
AU  - Bishop JM
FAU - Varmus, H E
AU  - Varmus HE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (DNA, Viral)
RN  - 0 (Nucleoproteins)
RN  - EC 3.1.- (Deoxyribonucleases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Centrifugation, Density Gradient
MH  - Chromatography, Gel
MH  - DNA, Viral/analysis/*genetics
MH  - Deoxyribonucleases/metabolism
MH  - Mice
MH  - Nucleoproteins/*physiology
MH  - Precipitin Tests
MH  - *Recombination, Genetic
MH  - Retroviridae/*genetics
MH  - Virus Replication
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
AID - 10.1101/gad.3.4.469 [doi]
PST - ppublish
SO  - Genes Dev. 1989 Apr;3(4):469-78. doi: 10.1101/gad.3.4.469.