PMID- 2746304
OWN - NLM
STAT- MEDLINE
DCOM- 19890814
LR  - 20190908
IS  - 0300-4864 (Print)
IS  - 0300-4864 (Linking)
VI  - 18
IP  - 3
DP  - 1989 Jun
TI  - Bromodeoxyuridine immunohistochemical determination of the lengths of the cell 
      cycle and the DNA-synthetic phase for an anatomically defined population.
PG  - 311-8
AB  - A cumulative labelling protocol using 5-bromo-2'-deoxyuridine (BUdR) was followed 
      to determine: (1) the growth fraction (i.e., the proportion of cells that 
      comprise the proliferating population), (2) the length of the cell cycle, and (3) 
      the length of the DNA-synthetic phase (S-phase) for proliferative cells in the 
      dentate gyrus of the mouse. On postnatal day 20 (P20), C57BL/6J mice were 
      injected with BUdR at two hour intervals for a total period of 12 hours. Animals 
      were sacrificed at selected intervals, and the brains were processed for 
      immunohistochemistry using a monoclonal antibody directed against single-stranded 
      DNA containing BUdR. The numbers of BUdR-labelled and unlabelled cells in 
      sections through the hilus of the dentate gyrus were counted. The number of 
      BUdR-labelled cells increased linearly from an initial value of about 12% of the 
      total number of cells to a maximum value of just over 24% of the total. These 
      findings indicate that, at P20, a maximum of 24.2 +/- 1.2% of the cells in the 
      dentate hilus are part of the proliferating population. The calculated length of 
      the cell cycle of the cells comprising the intrahilar proliferative zone was 
      estimated to be 16.1 +/- 0.8 h. The length of the S-phase was estimated at 8.0 
      +/- 0.4 h. In addition, mathematical analysis, using one and two population 
      models, indicates that over 90% of the proliferating cells in the dentate hilus 
      at this age comprise a single population at least in terms of the lengths of the 
      cell cycle and the S-phase.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Nowakowski, R S
AU  - Nowakowski RS
AD  - Department of Anatomy, UMDNJ-Robert Wood Johnson Medical School, Piscataway 
      08854.
FAU - Lewin, S B
AU  - Lewin SB
FAU - Miller, M W
AU  - Miller MW
LA  - eng
GR  - AA 06916/AA/NIAAA NIH HHS/United States
GR  - AA 07568/AA/NIAAA NIH HHS/United States
GR  - NS 23647/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurocytol
JT  - Journal of neurocytology
JID - 0364620
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - *Bromodeoxyuridine
MH  - *Cell Cycle
MH  - Hippocampus/cytology
MH  - Immunohistochemistry
MH  - *Interphase
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Time Factors
EDAT- 1989/06/01 00:00
MHDA- 1989/06/01 00:01
CRDT- 1989/06/01 00:00
PHST- 1989/06/01 00:00 [pubmed]
PHST- 1989/06/01 00:01 [medline]
PHST- 1989/06/01 00:00 [entrez]
AID - 10.1007/BF01190834 [doi]
PST - ppublish
SO  - J Neurocytol. 1989 Jun;18(3):311-8. doi: 10.1007/BF01190834.