PMID- 2766459
OWN - NLM
STAT- MEDLINE
DCOM- 19891012
LR  - 20190510
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 10
IP  - 9
DP  - 1989 Sep
TI  - Inactivation of O6-alkylguanine-DNA alkyltransferase in HeLa cells by cisplatin.
PG  - 1681-4
AB  - Inactivation of O6-alkylguanine-DNA alkyltransferase (O6-AGT) in HeLa CCL2 cells 
      by cisplatin was studied. HeLa CCL2 cells treated with cisplatin showed a 
      dose-dependent decline in O6-AGT activity. After cisplatin was removed and 
      replaced with fresh medium, the transferase level began to rise slowly. By 72 h 
      slightly more than 80% of the activity was recovered. It seems that the activity 
      of the alkyltransferase can be inactivated by platinated DNA adducts. The data 
      suggest that the O6-platinum-guanine formation and the O6-alkyltransferase 
      depletion are not responsible for cytotoxicity but may result in a base 
      substitution mutation in mammalian cells.
FAU - Wang, L G
AU  - Wang LG
AD  - Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Setlow, R B
AU  - Setlow RB
LA  - eng
PT  - Journal Article
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.63 (O(6)-Methylguanine-DNA Methyltransferase)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Cell Survival/drug effects
MH  - Cisplatin/*pharmacology
MH  - HeLa Cells/cytology/drug effects/enzymology
MH  - Humans
MH  - Kinetics
MH  - Methyltransferases/*antagonists & inhibitors/biosynthesis
MH  - O(6)-Methylguanine-DNA Methyltransferase
EDAT- 1989/09/01 00:00
MHDA- 1989/09/01 00:01
CRDT- 1989/09/01 00:00
PHST- 1989/09/01 00:00 [pubmed]
PHST- 1989/09/01 00:01 [medline]
PHST- 1989/09/01 00:00 [entrez]
AID - 10.1093/carcin/10.9.1681 [doi]
PST - ppublish
SO  - Carcinogenesis. 1989 Sep;10(9):1681-4. doi: 10.1093/carcin/10.9.1681.