PMID- 27799368
OWN - NLM
STAT- MEDLINE
DCOM- 20170531
LR  - 20240922
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 215
IP  - 3
DP  - 2016 Nov 7
TI  - Msi RNA-binding proteins control reserve intestinal stem cell quiescence.
PG  - 401-413
AB  - Regeneration of the intestinal epithelium is driven by multiple intestinal stem 
      cell (ISC) types, including an active, radiosensitive Wnt(high) ISC that fuels 
      turnover during homeostasis and a reserve, radioresistant Wnt(low/off) ISC 
      capable of generating active Wnt(high) ISCs. We examined the role of the Msi 
      family of oncoproteins in the ISC compartment. We demonstrated that Msi proteins 
      are dispensable for normal homeostasis and self-renewal of the active ISC, 
      despite their being highly expressed in these cells. In contrast, Msi proteins 
      are required specifically for activation of reserve ISCs, where Msi activity is 
      both necessary and sufficient to drive exit from quiescence and entry into the 
      cell cycle. Ablation of Msi activity in reserve ISCs rendered the epithelium 
      unable to regenerate in response to injury that ablates the active stem cell 
      compartment. These findings delineate a molecular mechanism governing reserve ISC 
      quiescence and demonstrate a necessity for the activity of this rare stem cell 
      population in intestinal regeneration.
CI  - (c) 2016 Yousefi et al.
FAU - Yousefi, Maryam
AU  - Yousefi M
AD  - Department of Biomedical Sciences, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104.
AD  - Cell and Molecular Biology Graduate Program, University of Pennsylvania, 
      Philadelphia, PA 19104.
FAU - Li, Ning
AU  - Li N
AD  - Department of Biomedical Sciences, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104.
FAU - Nakauka-Ddamba, Angela
AU  - Nakauka-Ddamba A
AUID- ORCID: 0000-0002-4631-7105
AD  - Department of Biomedical Sciences, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104.
FAU - Wang, Shan
AU  - Wang S
AUID- ORCID: 0000-0002-0429-8890
AD  - Department of Biomedical Sciences, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104.
AD  - State Key Laboratories for Agrobiotechnology, College of Biological Sciences, 
      China Agricultural University, Beijing 100083, China.
FAU - Davidow, Kimberly
AU  - Davidow K
AUID- ORCID: 0000-0003-0597-5475
AD  - Department of Biomedical Sciences, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104.
FAU - Schoenberger, Jenna
AU  - Schoenberger J
AUID- ORCID: 0000-0003-4278-669X
AD  - Department of Biomedical Sciences, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104.
FAU - Yu, Zhengquan
AU  - Yu Z
AUID- ORCID: 0000-0001-8696-2013
AD  - State Key Laboratories for Agrobiotechnology, College of Biological Sciences, 
      China Agricultural University, Beijing 100083, China.
FAU - Jensen, Shane T
AU  - Jensen ST
AD  - Department of Biostatistics, the Wharton School, University of Pennsylvania, 
      Philadelphia, PA 19104.
FAU - Kharas, Michael G
AU  - Kharas MG
AUID- ORCID: 0000-0002-1165-6991
AD  - Molecular Pharmacology and Chemistry Program, Experimental Therapeutics Center 
      and Center for Stem Cell Biology, Memorial Sloan-Kettering Cancer Center, New 
      York, NY 10065.
FAU - Lengner, Christopher J
AU  - Lengner CJ
AUID- ORCID: 0000-0002-0574-5189
AD  - Department of Biomedical Sciences, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104 lengner@vet.upenn.edu.
AD  - Department of Cell and Developmental Biology, School of Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104.
AD  - Cell and Molecular Biology Graduate Program, University of Pennsylvania, 
      Philadelphia, PA 19104.
AD  - Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 
      19104.
LA  - eng
GR  - P30 CA015704/CA/NCI NIH HHS/United States
GR  - P30 DK050306/DK/NIDDK NIH HHS/United States
GR  - R01 DK101989/DK/NIDDK NIH HHS/United States
GR  - R01 CA168654/CA/NCI NIH HHS/United States
GR  - R01 CA193842/CA/NCI NIH HHS/United States
GR  - R01 DK106309/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20161031
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Msi1h protein, mouse)
RN  - 0 (Msi2h protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Cell Lineage/radiation effects
MH  - Cell Proliferation/radiation effects
MH  - Epithelium/pathology/radiation effects
MH  - Gamma Rays
MH  - Homeostasis/radiation effects
MH  - Intestines/*cytology
MH  - Mice, Inbred C57BL
MH  - Nerve Tissue Proteins/*metabolism
MH  - RNA-Binding Proteins/*metabolism
MH  - Radiation Injuries/pathology
MH  - Resting Phase, Cell Cycle/radiation effects
MH  - S Phase/radiation effects
MH  - Stem Cells/*cytology/*metabolism/radiation effects
MH  - Up-Regulation/radiation effects
MH  - Wnt Signaling Pathway/radiation effects
PMC - PMC5100293
EDAT- 2016/11/02 06:00
MHDA- 2017/06/01 06:00
PMCR- 2017/05/07
CRDT- 2016/11/02 06:00
PHST- 2016/04/27 00:00 [received]
PHST- 2016/08/12 00:00 [revised]
PHST- 2016/09/29 00:00 [accepted]
PHST- 2016/11/02 06:00 [pubmed]
PHST- 2017/06/01 06:00 [medline]
PHST- 2016/11/02 06:00 [entrez]
PHST- 2017/05/07 00:00 [pmc-release]
AID - jcb.201604119 [pii]
AID - 201604119 [pii]
AID - 10.1083/jcb.201604119 [doi]
PST - ppublish
SO  - J Cell Biol. 2016 Nov 7;215(3):401-413. doi: 10.1083/jcb.201604119. Epub 2016 Oct 
      31.