PMID- 2808533 OWN - NLM STAT- MEDLINE DCOM- 19891205 LR - 20190508 IS - 0021-9525 (Print) IS - 1540-8140 (Electronic) IS - 0021-9525 (Linking) VI - 109 IP - 5 DP - 1989 Nov TI - Drosophila laminin: sequence of B2 subunit and expression of all three subunits during embryogenesis. PG - 2441-53 AB - In a previous study, we described the cloning of the genes encoding the three subunits of Drosophila laminin, a substrate adhesion molecule, and the cDNA sequence of the B1 subunit (Montell and Goodman, 1988). This analysis revealed the similarity of Drosophila laminin with the mouse and human complexes in subunit composition, domain structure, and amino acid sequence. In this paper, we report the deduced amino acid sequence of the B2 subunit. We then describe the expression and tissue distribution of the three subunits of laminin during Drosophila embryogenesis using both in situ hybridization and immunolocalization techniques, with particular emphasis on its expression in and around the developing nervous system. FAU - Montell, D J AU - Montell DJ AD - Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley 94720. FAU - Goodman, C S AU - Goodman CS LA - eng GR - NS18366/NS/NINDS NIH HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Cell Biol JT - The Journal of cell biology JID - 0375356 RN - 0 (Laminin) RN - 0 (Macromolecular Substances) RN - 9007-49-2 (DNA) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Cloning, Molecular MH - DNA/genetics MH - Drosophila/embryology/*genetics MH - Embryo, Nonmammalian/metabolism MH - *Gene Expression MH - *Genes MH - Laminin/*genetics MH - Macromolecular Substances MH - Mice MH - Molecular Sequence Data MH - Nucleic Acid Hybridization MH - Sequence Homology, Nucleic Acid MH - Species Specificity PMC - PMC2115843 EDAT- 1989/11/01 00:00 MHDA- 1989/11/01 00:01 PMCR- 1990/05/01 CRDT- 1989/11/01 00:00 PHST- 1989/11/01 00:00 [pubmed] PHST- 1989/11/01 00:01 [medline] PHST- 1989/11/01 00:00 [entrez] PHST- 1990/05/01 00:00 [pmc-release] AID - 90037237 [pii] AID - 10.1083/jcb.109.5.2441 [doi] PST - ppublish SO - J Cell Biol. 1989 Nov;109(5):2441-53. doi: 10.1083/jcb.109.5.2441.