PMID- 2824526
OWN - NLM
STAT- MEDLINE
DCOM- 19880107
LR  - 20220913
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 105
IP  - 5
DP  - 1987 Nov
TI  - Drosophila spectrin. II. Conserved features of the alpha-subunit are revealed by 
      analysis of cDNA clones and fusion proteins.
PG  - 2103-10
AB  - Drosophila alpha-spectrin cDNA sequences were isolated from a lambda gt11 
      expression library. These cDNA clones encode fusion proteins that include 
      portions of the Drosophila alpha-spectrin polypeptide as shown by a number of 
      structural and functional criteria. The fusion proteins elicited antibodies that 
      reacted strongly with Drosophila and vertebrate alpha-spectrins and a comparison 
      of cyanogen bromide peptide maps demonstrated a clear structural correspondence 
      between one fusion protein and purified Drosophila alpha-spectrin. Alpha-spectrin 
      fusion protein also displayed calcium-dependent calmodulin-binding activity in 
      blot overlay experiments and one fusion protein bound specifically to both 
      Drosophila and bovine brain beta-spectrin subunits on protein blots. A region of 
      the Drosophila cDNA cross-hybridized at lowered stringency with an avian 
      alpha-spectrin cDNA. Together these data show that the composition, structure, 
      and binding properties of the spectrin family of proteins have been remarkably 
      well conserved between arthropods and vertebrates. Drosophila cDNA hybridized to 
      an mRNA of greater than or equal to 9 kb on blots of total Drosophila poly A+ 
      RNA; and hybridized in situ to a single site in polytene region 62B, 1-7. This 
      result and Southern blot analysis of genomic DNA indicate that the sequences are 
      likely to be single copy in the Drosophila genome.
FAU - Byers, T J
AU  - Byers TJ
AD  - Department of Cellular and Developmental Biology, Harvard University, Cambridge, 
      Massachusetts 02138.
FAU - Dubreuil, R
AU  - Dubreuil R
FAU - Branton, D
AU  - Branton D
FAU - Kiehart, D P
AU  - Kiehart DP
FAU - Goldstein, L S
AU  - Goldstein LS
LA  - eng
GR  - GM-33830/GM/NIGMS NIH HHS/United States
GR  - GM-35252/GM/NIGMS NIH HHS/United States
GR  - HL-17411/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Macromolecular Substances)
RN  - 0 (Recombinant Proteins)
RN  - 12634-43-4 (Spectrin)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - *Cloning, Molecular
MH  - DNA/*analysis
MH  - DNA Restriction Enzymes
MH  - Drosophila/*genetics
MH  - *Genes
MH  - Macromolecular Substances
MH  - Nucleic Acid Hybridization
MH  - Recombinant Proteins/isolation & purification
MH  - Spectrin/*genetics/isolation & purification
PMC - PMC2114868
EDAT- 1987/11/01 00:00
MHDA- 1987/11/01 00:01
PMCR- 1988/05/01
CRDT- 1987/11/01 00:00
PHST- 1987/11/01 00:00 [pubmed]
PHST- 1987/11/01 00:01 [medline]
PHST- 1987/11/01 00:00 [entrez]
PHST- 1988/05/01 00:00 [pmc-release]
AID - 88059243 [pii]
AID - 10.1083/jcb.105.5.2103 [doi]
PST - ppublish
SO  - J Cell Biol. 1987 Nov;105(5):2103-10. doi: 10.1083/jcb.105.5.2103.