PMID- 2847923
OWN - NLM
STAT- MEDLINE
DCOM- 19890109
LR  - 20191022
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 12
IP  - 4
DP  - 1988
TI  - Induction of chromosome aberrations and mitotic arrest by cytomegalovirus in 
      human cells.
PG  - 409-20
AB  - Human cytomegalovirus (CMV) is potentially an effective but often overlooked 
      genotoxic agent in humans. We report here evidence that indicates that infection 
      by CMV can induce chromosome alterations and mitotic inhibition. The frequency of 
      chromosome aberrations induced was dependent on the input multiplicity of 
      infection (m.o.i.) for human lung fibroblasts (LU), but not for human peripheral 
      blood lymphocytes (PBLs) when both cell types were infected at the GO phase of 
      the cell cycle. The aberrations induced by CMV were mostly chromatid breaks and 
      chromosome pulverizations that resembled prematurely condensed S-phase chromatin. 
      Pulverized chromosomes were not observed in LU cells infected with virus stocks 
      that had been rendered nonlytic by UV-irradiation at 24,000 ergs/mm2 or from 
      infection of human lymphocytes. In LU cells infected with UV-irradiated CMV, the 
      frequency of aberrations induced was inversely dependent on the extent of the 
      exposure of the CMV stock to the UV-light. In permissive CMV infection of 
      proliferating LU cells at 24 hr after subculture, a high percentage (greater than 
      40%) of the metaphase cells were arrested at their first metaphase and displayed 
      severely condensed chromosomes when harvested 48 hr later. A significant increase 
      (p less than 0.05) in the chromosome aberration frequency was also observed. Our 
      study shows that CMV infection is genotoxic to host cells. The types and extent 
      of damage are dependent on the viral genome expression and on the cell cycle 
      stage of the cells at the time of infection. The possible mechanisms for 
      induction of chromosome damage by CMV are discussed.
FAU - AbuBakar, S
AU  - AbuBakar S
AD  - Department of Microbiology, University of Texas Medical Branch, Galveston 77550.
FAU - Au, W W
AU  - Au WW
FAU - Legator, M S
AU  - Legator MS
FAU - Albrecht, T
AU  - Albrecht T
LA  - eng
GR  - 1 AI 42 557/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
SB  - IM
MH  - Cell Division
MH  - *Cell Transformation, Viral
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Cytomegalovirus/*physiology/radiation effects
MH  - Cytopathogenic Effect, Viral
MH  - Female
MH  - Fibroblasts
MH  - Humans
MH  - *Mitosis
MH  - Mitotic Index
MH  - Ultraviolet Rays
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
AID - 10.1002/em.2860120409 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 1988;12(4):409-20. doi: 10.1002/em.2860120409.