PMID- 2863759
OWN - NLM
STAT- MEDLINE
DCOM- 19851011
LR  - 20190617
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 316
IP  - 6031
DP  - 1985 Aug 29-Sep 4
TI  - Amplification of P-glycoprotein genes in multidrug-resistant mammalian cell 
      lines.
PG  - 817-9
AB  - The multidrug-resistance phenotype expressed in mammalian cell lines is complex. 
      Cells selected with a single agent can acquire cross-resistance to a remarkably 
      wide range of compounds which have no obvious structural or functional 
      similarities. The basis for cross-resistance seems to be a decreased net cellular 
      accumulation of the drug involved, and has been attributed to alterations in the 
      plasma membrane. An over-expressed plasma membrane glycoprotein of relative 
      molecular mass (Mr) 170,000 (P-glycoprotein) is consistently found in different 
      multidrug-resistant human and animal cell lines, and in transplantable tumours. 
      Consequently, it has been postulated that P-glycoprotein directly or indirectly 
      mediates multidrug resistance. Here we report the cloning of a complementary DNA 
      encoding P-glycoprotein. Southern blot analysis of hamster, mouse and human DNA 
      using this cDNA as a probe showed that P-glycoprotein is conserved and is 
      probably encoded by a gene family, and that members of this putative family are 
      amplified in multidrug-resistant cells.
FAU - Riordan, J R
AU  - Riordan JR
FAU - Deuchars, K
AU  - Deuchars K
FAU - Kartner, N
AU  - Kartner N
FAU - Alon, N
AU  - Alon N
FAU - Trent, J
AU  - Trent J
FAU - Ling, V
AU  - Ling V
LA  - eng
GR  - CA-17094/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Glycoproteins)
RN  - 9007-49-2 (DNA)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA/metabolism
MH  - Drug Resistance
MH  - Female
MH  - *Gene Amplification
MH  - *Genes
MH  - Glycoproteins/*genetics
MH  - Nucleic Acid Hybridization
MH  - Ovary
MH  - beta-Galactosidase/genetics
EDAT- 1985/08/04 00:00
MHDA- 1985/08/04 00:01
CRDT- 1985/08/04 00:00
PHST- 1985/08/04 00:00 [pubmed]
PHST- 1985/08/04 00:01 [medline]
PHST- 1985/08/04 00:00 [entrez]
AID - 10.1038/316817a0 [doi]
PST - ppublish
SO  - Nature. 1985 Aug 29-Sep 4;316(6031):817-9. doi: 10.1038/316817a0.