PMID- 28689984 OWN - NLM STAT- MEDLINE DCOM- 20170914 LR - 20191008 IS - 1097-4199 (Electronic) IS - 0896-6273 (Print) IS - 0896-6273 (Linking) VI - 95 IP - 2 DP - 2017 Jul 19 TI - Local Cues Establish and Maintain Region-Specific Phenotypes of Basal Ganglia Microglia. PG - 341-356.e6 LID - S0896-6273(17)30518-4 [pii] LID - 10.1016/j.neuron.2017.06.020 [doi] AB - Microglia play critical roles in tissue homeostasis and can also modulate neuronal function and synaptic connectivity. In contrast to astrocytes and oligodendrocytes, which arise from multiple progenitor pools, microglia arise from yolk sac progenitors and are widely considered to be equivalent throughout the CNS. However, little is known about basic properties of deep brain microglia, such as those within the basal ganglia (BG). Here, we show that microglial anatomical features, lysosome content, membrane properties, and transcriptomes differ significantly across BG nuclei. Region-specific phenotypes of BG microglia emerged during the second postnatal week and were re-established following genetic or pharmacological microglial ablation and repopulation in the adult, indicating that local cues play an ongoing role in shaping microglial diversity. These findings demonstrate that microglia in the healthy brain exhibit a spectrum of distinct functional states and provide a critical foundation for defining microglial contributions to BG circuit function. CI - Published by Elsevier Inc. FAU - De Biase, Lindsay M AU - De Biase LM AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. Electronic address: lindsay.debiase@nih.gov. FAU - Schuebel, Kornel E AU - Schuebel KE AD - Intramural Research Program, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD 20852, USA. FAU - Fusfeld, Zachary H AU - Fusfeld ZH AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. FAU - Jair, Kamwing AU - Jair K AD - Intramural Research Program, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD 20852, USA. FAU - Hawes, Isobel A AU - Hawes IA AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. FAU - Cimbro, Raffaello AU - Cimbro R AD - Division of Rheumatology, Bayview Flow Cytometry Core, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA. FAU - Zhang, Hai-Ying AU - Zhang HY AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. FAU - Liu, Qing-Rong AU - Liu QR AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. FAU - Shen, Hui AU - Shen H AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. FAU - Xi, Zheng-Xiong AU - Xi ZX AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. FAU - Goldman, David AU - Goldman D AD - Intramural Research Program, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD 20852, USA. FAU - Bonci, Antonello AU - Bonci A AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. Electronic address: antonello.bonci@nih.gov. LA - eng GR - ZIA DA000596-02/Intramural NIH HHS/United States GR - ZIA DA000596-04/Intramural NIH HHS/United States GR - ZIA DA000596-01/Intramural NIH HHS/United States GR - P30 AR070254/AR/NIAMS NIH HHS/United States GR - ZIA DA000596-03/Intramural NIH HHS/United States PT - Journal Article DEP - 20170706 PL - United States TA - Neuron JT - Neuron JID - 8809320 SB - IM MH - Animals MH - Basal Ganglia/pathology/*physiology MH - Cues MH - Mice, Transgenic MH - Microglia/*metabolism MH - Neurons/physiology MH - Phenotype PMC - PMC5754189 MID - NIHMS890085 OTO - NOTNLM OT - RNA sequencing OT - density OT - development OT - electrophysiology OT - heterogeneity OT - microglia OT - morphology OT - nucleus accumbens OT - substantia nigra OT - ventral tegmental area EDAT- 2017/07/12 06:00 MHDA- 2017/09/15 06:00 PMCR- 2018/07/19 CRDT- 2017/07/11 06:00 PHST- 2016/08/23 00:00 [received] PHST- 2017/04/13 00:00 [revised] PHST- 2017/06/12 00:00 [accepted] PHST- 2017/07/12 06:00 [pubmed] PHST- 2017/09/15 06:00 [medline] PHST- 2017/07/11 06:00 [entrez] PHST- 2018/07/19 00:00 [pmc-release] AID - S0896-6273(17)30518-4 [pii] AID - 10.1016/j.neuron.2017.06.020 [doi] PST - ppublish SO - Neuron. 2017 Jul 19;95(2):341-356.e6. doi: 10.1016/j.neuron.2017.06.020. Epub 2017 Jul 6.