PMID- 2888312
OWN - NLM
STAT- MEDLINE
DCOM- 19871002
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 128
IP  - 3
DP  - 1987 Sep
TI  - Differentiation of human neuroblastoma recapitulates neural crest development. 
      Study of morphology, neurotransmitter enzymes, and extracellular matrix proteins.
PG  - 484-96
AB  - Differentiation of human neuroblastoma (NB) was studied in vitro with five NB 
      cell lines treated with dibutyryl cyclic adenosinemonophosphate and retinoic 
      acid. Although the above agents induced different responses in the various cell 
      lines, three overall morphologic phenotypes emerged: a neuronal, characterized by 
      cell processes and neurosecretory granules, a flat cell without pigment, which 
      displayed basal lamina pertinent to Schwann cells, and a flat pigmented cell 
      which exhibited melanosomes, similarly to melanocytes. The activity of the 
      Schwann cell enzyme cyclic nucleotidyl phosphohydrolase increased considerably in 
      one condition, after induction of a predominantly flat cell phenotype. All 
      studied NB cell lines were capable of synthesizing and expressing the 
      extracellular matrix proteins laminin (LM), fibronectin (FN), and Type IV 
      collagen; but a specific pattern of expression emerged after differentiation, 
      which was proportional to normal tissue equivalents: neuronal--none; 
      melanocytic--FN only; and Schwann cell--large amounts of FN, LM, and Type IV 
      collagen.
FAU - Tsokos, M
AU  - Tsokos M
FAU - Scarpa, S
AU  - Scarpa S
FAU - Ross, R A
AU  - Ross RA
FAU - Triche, T J
AU  - Triche TJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Fibronectins)
RN  - 0 (Laminin)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (S100 Proteins)
RN  - 5688UTC01R (Tretinoin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - EC 3.1.4.- (2',3'-Cyclic-Nucleotide Phosphodiesterases)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.37 (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase)
RN  - EC 3.1.4.37 (CNP protein, human)
SB  - IM
MH  - 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
MH  - 2',3'-Cyclic-Nucleotide Phosphodiesterases/metabolism
MH  - Bucladesine/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Choline O-Acetyltransferase/metabolism
MH  - Collagen/biosynthesis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Extracellular Matrix/*metabolism
MH  - Fibronectins/biosynthesis
MH  - Humans
MH  - Laminin/biosynthesis
MH  - Melanocytes/pathology
MH  - Microscopy, Electron
MH  - Neural Crest/*physiology
MH  - Neuroblastoma/metabolism/*pathology
MH  - Neurons/pathology
MH  - Neurotransmitter Agents/*metabolism
MH  - *Phosphoric Diester Hydrolases
MH  - *Protein Biosynthesis
MH  - S100 Proteins/biosynthesis
MH  - Schwann Cells/pathology
MH  - Tretinoin/pharmacology
PMC - PMC1899665
EDAT- 1987/09/01 00:00
MHDA- 1987/09/01 00:01
PMCR- 1988/03/01
CRDT- 1987/09/01 00:00
PHST- 1987/09/01 00:00 [pubmed]
PHST- 1987/09/01 00:01 [medline]
PHST- 1987/09/01 00:00 [entrez]
PHST- 1988/03/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1987 Sep;128(3):484-96.