PMID- 2891460
OWN - NLM
STAT- MEDLINE
DCOM- 19880217
LR  - 20190820
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 46
IP  - 1
DP  - 1988 Jan
TI  - Antibody-directed affinity therapy applied to the immune system: in vivo 
      effectiveness and limited toxicity of daunomycin conjugated to HPMA copolymers 
      and targeting antibody.
PG  - 100-14
AB  - The applicability of targeting therapy intervention in lymphatic tissue was 
      studied. The effect was measured as the inhibition of anti-sheep red blood cell 
      antibody response expressed in plaque-forming cells. Daunomycin was used as the 
      effective drug and polyclonal and monoclonal anti-Thy 1.2 or anti-Iak antibody 
      served for targeting. Both components were coupled to a soluble 
      N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer with oligopeptidic side 
      sequences which permitted a controlled release of the drug in the target tissue. 
      HPMA copolymer conjugates with side sequences Gly-Phe-Leu-Gly cleavable by 
      lysosomal enzymes decreased in vivo the antibody reaction by 60-85%. A comparable 
      amount of free targeting antibody was without a significant effect. Injection of 
      targeted daunomycin decreased the toxicity of the drug against hematopoietic 
      precursors in bone marrow colony-forming unit-spleen 80 times compared to the 
      same amount of free drug. The in vivo effectiveness of targeted daunomycin was 
      confirmed morphologically. Application of free daunomycin lead to a significant 
      irritation of Kupffer cells in liver while none of the 
      daunomycin-antibody-copolymer conjugate had such an effect.
FAU - Rihova, B
AU  - Rihova B
AD  - Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
FAU - Kopeckova, P
AU  - Kopeckova P
FAU - Strohalm, J
AU  - Strohalm J
FAU - Rossmann, P
AU  - Rossmann P
FAU - Vetvicka, V
AU  - Vetvicka V
FAU - Kopecek, J
AU  - Kopecek J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Acrylates)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Methacrylates)
RN  - 0 (Thy-1 Antigens)
RN  - UKW89XAX2X (hydroxypropyl methacrylate)
RN  - ZS7284E0ZP (Daunorubicin)
SB  - IM
MH  - Acrylates/*administration & dosage
MH  - Animals
MH  - Antibodies, Monoclonal/*administration & dosage
MH  - Antigens, Surface/immunology
MH  - Colony-Forming Units Assay
MH  - Daunorubicin/administration & dosage/*pharmacokinetics/toxicity
MH  - Female
MH  - Hematopoietic Stem Cells/drug effects
MH  - Histocompatibility Antigens Class II/immunology
MH  - Methacrylates/*administration & dosage
MH  - Mice
MH  - Mice, Inbred A
MH  - Spleen/cytology
MH  - Thy-1 Antigens
MH  - Tissue Distribution
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
AID - 10.1016/0090-1229(88)90010-4 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1988 Jan;46(1):100-14. doi: 
      10.1016/0090-1229(88)90010-4.