PMID- 2904703
OWN - NLM
STAT- MEDLINE
DCOM- 19890126
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 242
IP  - 4886
DP  - 1988 Dec 23
TI  - Engraftment of immune-deficient mice with human hematopoietic stem cells.
PG  - 1706-9
AB  - A system in which immune-deficient mice are repopulated with cells from the human 
      myeloid lineage, and that provides an in vivo stem cell assay for human 
      hematopoietic cells is described. Generation of the chimeric 
      human/immune-deficient (HID) mice was dependent on the use of immune-deficient 
      bg/nu/xid mice. Infusion of these mice with human bone marrow gave rise to 
      increases in human macrophage progenitors during more than 5 weeks of in vivo 
      growth, indicating the seeding, proliferation, and differentiation of human stem 
      cells. The human identity of the progenitors was confirmed by sequence analysis 
      and their dependence on human growth factors. The creation of HID mice lays the 
      foundation for establishing animal models for a wide variety of human 
      hemopathies, from leukemia to infectious disease.
FAU - Kamel-Reid, S
AU  - Kamel-Reid S
AD  - Department of Genetics, Hospital for Sick Children, Toronto, Ontario.
FAU - Dick, J E
AU  - Dick JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Interleukin-3)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - Cell Division
MH  - Colony-Forming Units Assay
MH  - Colony-Stimulating Factors/pharmacology
MH  - Disease Models, Animal
MH  - Granulocyte-Macrophage Colony-Stimulating Factor
MH  - Growth Substances/pharmacology
MH  - Hematologic Diseases
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/pathology
MH  - Humans
MH  - *Immunologic Deficiency Syndromes/immunology/pathology
MH  - Interleukin-3/pharmacology
MH  - Killer Cells, Natural/immunology
MH  - Macrophages/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Mice, Mutant Strains
MH  - Mice, Nude
MH  - Transplantation, Heterologous
EDAT- 1988/12/23 00:00
MHDA- 1988/12/23 00:01
CRDT- 1988/12/23 00:00
PHST- 1988/12/23 00:00 [pubmed]
PHST- 1988/12/23 00:01 [medline]
PHST- 1988/12/23 00:00 [entrez]
AID - 10.1126/science.2904703 [doi]
PST - ppublish
SO  - Science. 1988 Dec 23;242(4886):1706-9. doi: 10.1126/science.2904703.