PMID- 29552200
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220321
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 4
DP  - 2018 Apr
TI  - CD44 promotes cell proliferation in non-small cell lung cancer.
PG  - 5627-5633
LID - 10.3892/ol.2018.8051 [doi]
AB  - Cluster of differentiation 44 (CD44), a marker for cancer stem cells, has been 
      reported to be associated with poor prognosis in non-small cell lung cancer 
      (NSCLC), but its involvement in tumor growth has not fully been elucidated. The 
      present study explored the associations between CD44 expression and 
      clinicopathological features using immunohistochemistry in 94 NSCLC cases, as 
      well as proliferation in cells with aberrant expression of this protein. 
      Overexpression of CD44 was achieved by transfecting H1299 cells with CD44 
      expression vectors, and inhibition of CD44 expression was performed by 
      transfecting small interfering RNAs into A549 cells. Cell proliferation was 
      measured using Cell Counting Kit-8 assays and flat plate colony formation assay 
      was performed to confirm the cellular anchorage growth in vitro. In total, 64 
      (68.1%) of the 94 NSCLC cases stained positive for CD44 expression. High 
      expression of CD44 was associated with advanced T stage in NSCLC. Overexpression 
      of CD44 in H1299 cells promoted cell proliferation and colony formation in vitro. 
      Meanwhile, knockdown of CD44 expression in A549 cells suppressed cell 
      proliferation and colony formation in vitro. High expression of CD44 may promote 
      NSCLC progression by increasing cancer cell proliferation; therefore, it may 
      serve as a potential biomarker for diagnosis or target therapy.
FAU - Hu, Bo
AU  - Hu B
AD  - Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and 
      Translational Research (Ministry of Education/Beijing), Peking University Cancer 
      Hospital and Institute, Beijing 100142, P.R. China.
FAU - Ma, Yuanyuan
AU  - Ma Y
AD  - Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and 
      Translational Research (Ministry of Education/Beijing), Peking University Cancer 
      Hospital and Institute, Beijing 100142, P.R. China.
FAU - Yang, Yue
AU  - Yang Y
AD  - Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and 
      Translational Research (Ministry of Education/Beijing), Peking University Cancer 
      Hospital and Institute, Beijing 100142, P.R. China.
FAU - Zhang, Lijian
AU  - Zhang L
AD  - Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and 
      Translational Research (Ministry of Education/Beijing), Peking University Cancer 
      Hospital and Institute, Beijing 100142, P.R. China.
FAU - Han, Haibo
AU  - Han H
AD  - Department of Biobank, Key Laboratory of Carcinogenesis and Translational 
      Research (Ministry of Education/Beijing), Peking University Cancer Hospital and 
      Institute, Beijing 100142, P.R. China.
FAU - Chen, Jinfeng
AU  - Chen J
AD  - Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and 
      Translational Research (Ministry of Education/Beijing), Peking University Cancer 
      Hospital and Institute, Beijing 100142, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180214
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5840516
OTO - NOTNLM
OT  - T stage
OT  - cell proliferation
OT  - cluster of differentiation 44
OT  - knockdown
OT  - non-small cell lung cancer
OT  - overexpression
EDAT- 2018/03/20 06:00
MHDA- 2018/03/20 06:01
PMCR- 2018/02/14
CRDT- 2018/03/20 06:00
PHST- 2017/02/08 00:00 [received]
PHST- 2017/10/26 00:00 [accepted]
PHST- 2018/03/20 06:00 [entrez]
PHST- 2018/03/20 06:00 [pubmed]
PHST- 2018/03/20 06:01 [medline]
PHST- 2018/02/14 00:00 [pmc-release]
AID - OL-0-0-8051 [pii]
AID - 10.3892/ol.2018.8051 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Apr;15(4):5627-5633. doi: 10.3892/ol.2018.8051. Epub 2018 Feb 
      14.