PMID- 3012781
OWN - NLM
STAT- MEDLINE
DCOM- 19860708
LR  - 20220409
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 232
IP  - 4758
DP  - 1986 Jun 27
TI  - The product of the human c-erbB-2 gene: a 185-kilodalton glycoprotein with 
      tyrosine kinase activity.
PG  - 1644-6
AB  - Antibodies were raised against a synthetic peptide corresponding to 14 amino acid 
      residues at the COOH-terminus of a protein deduced from the human c-erbB-2 
      nucleotide sequence. These antibodies immunoprecipitated a 185-kilodalton 
      glycoprotein from MKN-7 adenocarcinoma cells. Incubation of the 
      immunoprecipitates with (gamma-32P)ATP resulted in the phosphorylation of this 
      protein on tyrosine residues. These results indicate that the human c-erbB-2 gene 
      product is the 185-kilodalton glycoprotein that is associated with tyrosine 
      kinase activity. Although the c-erbB-2 protein was predicted to encode a protein 
      very similar to epidermal growth factor (EGF) receptor, EGF did not stimulate 
      this kinase activity either in vivo or in vitro.
FAU - Akiyama, T
AU  - Akiyama T
FAU - Sudo, C
AU  - Sudo C
FAU - Ogawara, H
AU  - Ogawara H
FAU - Toyoshima, K
AU  - Toyoshima K
FAU - Yamamoto, T
AU  - Yamamoto T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Epidermal Growth Factor/metabolism
MH  - ErbB Receptors
MH  - *Genes
MH  - Glycoproteins/genetics/isolation & purification/*metabolism
MH  - HeLa Cells/metabolism
MH  - Humans
MH  - Molecular Weight
MH  - Oncogenes
MH  - Phosphorylation
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Receptors, Cell Surface/metabolism
EDAT- 1986/06/27 00:00
MHDA- 1986/06/27 00:01
CRDT- 1986/06/27 00:00
PHST- 1986/06/27 00:00 [pubmed]
PHST- 1986/06/27 00:01 [medline]
PHST- 1986/06/27 00:00 [entrez]
AID - 10.1126/science.3012781 [doi]
PST - ppublish
SO  - Science. 1986 Jun 27;232(4758):1644-6. doi: 10.1126/science.3012781.