PMID- 30270040
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20190729
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Linking)
VI  - 175
IP  - 4
DP  - 2018 Nov 1
TI  - Spatial Reconstruction of Single Enterocytes Uncovers Broad Zonation along the 
      Intestinal Villus Axis.
PG  - 1156-1167.e15
LID - S0092-8674(18)31164-4 [pii]
LID - 10.1016/j.cell.2018.08.063 [doi]
AB  - The intestinal epithelium is a highly structured tissue composed of repeating 
      crypt-villus units. Enterocytes perform the diverse tasks of absorbing a wide 
      range of nutrients while protecting the body from the harsh bacterium-rich 
      environment. It is unknown whether these tasks are spatially zonated along the 
      villus axis. Here, we extracted a large panel of landmark genes characterized by 
      transcriptomics of laser capture microdissected villus segments and utilized it 
      for single-cell spatial reconstruction, uncovering broad zonation of enterocyte 
      function along the villus. We found that enterocytes at villus bottoms express an 
      anti-bacterial gene program in a microbiome-dependent manner. They next shift to 
      sequential expression of carbohydrates, peptides, and fat absorption machineries 
      in distinct villus compartments. Finally, they induce a Cd73 immune-modulatory 
      program at the villus tips. Our approach can be used to uncover zonation patterns 
      in other organs when prior knowledge of landmark genes is lacking.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Moor, Andreas E
AU  - Moor AE
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 
      Israel. Electronic address: andreas.moor@weizmann.ac.il.
FAU - Harnik, Yotam
AU  - Harnik Y
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 
      Israel.
FAU - Ben-Moshe, Shani
AU  - Ben-Moshe S
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 
      Israel.
FAU - Massasa, Efi E
AU  - Massasa EE
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 
      Israel.
FAU - Rozenberg, Milena
AU  - Rozenberg M
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 
      Israel.
FAU - Eilam, Raya
AU  - Eilam R
AD  - Department of Veterinary Resources, Weizmann Institute of Science, Rehovot, 
      Israel.
FAU - Bahar Halpern, Keren
AU  - Bahar Halpern K
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 
      Israel.
FAU - Itzkovitz, Shalev
AU  - Itzkovitz S
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 
      Israel. Electronic address: shalev.itzkovitz@weizmann.ac.il.
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180927
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
CIN - Nat Rev Gastroenterol Hepatol. 2018 Dec;15(12):717. doi: 
      10.1038/s41575-018-0080-z. PMID: 30361614
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Enterocytes/cytology/*metabolism/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Single-Cell Analysis
MH  - *Transcriptome
OTO - NOTNLM
OT  - metabolism
OT  - mucosal immunology
OT  - single-cell transcriptomics
OT  - spatial transcriptomics
OT  - systems biology
EDAT- 2018/10/03 06:00
MHDA- 2019/07/30 06:00
CRDT- 2018/10/02 06:00
PHST- 2018/05/28 00:00 [received]
PHST- 2018/07/29 00:00 [revised]
PHST- 2018/08/29 00:00 [accepted]
PHST- 2018/10/03 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2018/10/02 06:00 [entrez]
AID - S0092-8674(18)31164-4 [pii]
AID - 10.1016/j.cell.2018.08.063 [doi]
PST - ppublish
SO  - Cell. 2018 Nov 1;175(4):1156-1167.e15. doi: 10.1016/j.cell.2018.08.063. Epub 2018 
      Sep 27.