PMID- 3098436
OWN - NLM
STAT- MEDLINE
DCOM- 19870218
LR  - 20231213
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 48
IP  - 1
DP  - 1987 Jan 16
TI  - Functional domains of Pseudomonas exotoxin identified by deletion analysis of the 
      gene expressed in E. coli.
PG  - 129-36
AB  - Pseudomonas exotoxin A is a single chain toxin with three structural domains that 
      inhibits protein synthesis in eukaryotic cells by catalyzing ADP ribosylation of 
      elongation factor 2. To study the function of these domains, we deleted different 
      portions of the PE structural gene and expressed these constructs in E. coli 
      using an inducible T7 promoter. These studies indicate that structural domain Ia 
      is required for cell recognition, that structural domain II is required to 
      translocate the toxin across a cellular membrane, and that structural domain III 
      and a portion of domain Ib are required for ADP ribosylation activity. Toxin 
      lacking domain Ia is about 100-fold less toxic to mice than intact PE and should 
      be a useful molecule for the construction of immunotoxins.
FAU - Hwang, J
AU  - Hwang J
FAU - Fitzgerald, D J
AU  - Fitzgerald DJ
FAU - Adhya, S
AU  - Adhya S
FAU - Pastan, I
AU  - Pastan I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Bacterial Toxins)
RN  - 0 (Exotoxins)
RN  - 0 (Virulence Factors)
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
RN  - EC 2.4.2.- (ADP Ribose Transferases)
RN  - EC 2.4.2.- (Pentosyltransferases)
SB  - IM
MH  - ADP Ribose Transferases
MH  - Adenosine Diphosphate Ribose/metabolism
MH  - *Bacterial Toxins
MH  - Binding Sites
MH  - Biological Transport
MH  - Chromosome Deletion
MH  - Cloning, Molecular
MH  - Escherichia coli
MH  - Exotoxins/*genetics/toxicity
MH  - Pentosyltransferases/metabolism
MH  - Pseudomonas aeruginosa/*pathogenicity
MH  - Structure-Activity Relationship
MH  - *Virulence Factors
MH  - Pseudomonas aeruginosa Exotoxin A
EDAT- 1987/01/16 00:00
MHDA- 1987/01/16 00:01
CRDT- 1987/01/16 00:00
PHST- 1987/01/16 00:00 [pubmed]
PHST- 1987/01/16 00:01 [medline]
PHST- 1987/01/16 00:00 [entrez]
AID - 0092-8674(87)90363-1 [pii]
AID - 10.1016/0092-8674(87)90363-1 [doi]
PST - ppublish
SO  - Cell. 1987 Jan 16;48(1):129-36. doi: 10.1016/0092-8674(87)90363-1.