PMID- 3182782
OWN - NLM
STAT- MEDLINE
DCOM- 19881202
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 263
IP  - 31
DP  - 1988 Nov 5
TI  - Purification and identification of [hydroxyprolyl3]bradykinin in ascitic fluid 
      from a patient with gastric cancer.
PG  - 16051-4
AB  - Kinins in the ascitic fluid from a patient with gastric cancer were purified by 
      gel filtration and reversed-phase high-performance liquid chromatography (HPLC). 
      Two fractions (fractions I and II) showed kinin activity. Fraction I did not 
      correspond to either bradykinin or other known kinins, whereas fraction II 
      corresponded to bradykinin. Fraction I contained 8 amino acid residues from 
      bradykinin minus 1 proline plus 1 additional hydroxyproline. Sequence analysis of 
      fraction I showed that the proline at the third amino acid residue of bradykinin 
      was replaced by hydroxyproline. The retention time of fraction I on 
      reversed-phase HPLC was exactly the same as that of synthetic 
      [hydroxyprolyl3]bradykinin (Arg-Pro-Hyp-Gly-Phe-Ser-Pro-Phe-Arg) and was 
      distinguishable from des-Pro3-bradykinin. Thus, these results demonstrate for the 
      first time the presence of [hydroxyprolyl3]bradykinin in vivo. This is also the 
      first report of the presence of bradykinin in human tumor ascites.
FAU - Maeda, H
AU  - Maeda H
AD  - Department of Microbiology, Kumamoto University Medical School, Japan.
FAU - Matsumura, Y
AU  - Matsumura Y
FAU - Kato, H
AU  - Kato H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amino Acids)
RN  - 0 (Kinins)
RN  - 37642-65-2 (bradykinin, hydroxy-Pro(3)-)
RN  - S8TIM42R2W (Bradykinin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acids/analysis
MH  - Ascites/*metabolism
MH  - Bradykinin/*analogs & derivatives/isolation & purification
MH  - Chromatography, Gel
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Kinins/isolation & purification
MH  - Molecular Sequence Data
MH  - Stomach Neoplasms/*metabolism
EDAT- 1988/11/05 00:00
MHDA- 1988/11/05 00:01
CRDT- 1988/11/05 00:00
PHST- 1988/11/05 00:00 [pubmed]
PHST- 1988/11/05 00:01 [medline]
PHST- 1988/11/05 00:00 [entrez]
AID - S0021-9258(18)37555-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1988 Nov 5;263(31):16051-4.