PMID- 3335311
OWN - NLM
STAT- MEDLINE
DCOM- 19880220
LR  - 20220225
IS  - 0016-5085 (Print)
IS  - 0016-5085 (Linking)
VI  - 94
IP  - 2
DP  - 1988 Feb
TI  - Localization of villin, a cytoskeletal protein specific to microvilli, in human 
      ileum and colon and in colonic neoplasms.
PG  - 343-52
AB  - Villin is a cytoskeletal protein of microvilli of epithelial cell brush borders 
      found principally in absorptive cells of the intestine and proximal renal tubule. 
      A marker of both enterocyte differentiation and epithelial cell polarity, it has 
      been studied mainly in experimental animals. We raised monoclonal antibodies to 
      villin and used them to localize it in human ileum and colon and in 22 colonic 
      neoplasms. Villin is localized in the brush border of normal ileum and in the 
      luminal border of normal colon and is expressed with increasing staining 
      intensity as cells migrate from crypt to surface. It was present in the luminal 
      border in all five adenomas and in 16 of 17 adenocarcinomas studied. In addition, 
      villin staining was observed in the cytoplasm of 10 tumors, and in the basement 
      membrane area surrounding tumor in 10 cases. In "transitional" mucosa adjacent to 
      carcinomas it was confined to the luminal border. Abnormal expression of villin 
      by a significant proportion of colonic tumors suggests that it may have a role as 
      a marker of colorectal neoplasia.
FAU - West, A B
AU  - West AB
AD  - Department of Pathology, Yale University, New Haven, Connecticut.
FAU - Isaac, C A
AU  - Isaac CA
FAU - Carboni, J M
AU  - Carboni JM
FAU - Morrow, J S
AU  - Morrow JS
FAU - Mooseker, M S
AU  - Mooseker MS
FAU - Barwick, K W
AU  - Barwick KW
LA  - eng
GR  - DK 25387/DK/NIDDK NIH HHS/United States
GR  - HL 28560/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Carrier Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (villin)
SB  - IM
MH  - Adenocarcinoma/metabolism
MH  - Adenoma/metabolism
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Basement Membrane/metabolism
MH  - Carrier Proteins/*metabolism
MH  - Chickens
MH  - Colon/*metabolism
MH  - Colonic Neoplasms/*metabolism
MH  - Female
MH  - Humans
MH  - Ileum/*metabolism
MH  - Immunoassay
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/metabolism
MH  - Male
MH  - Microfilament Proteins/*metabolism
MH  - Microvilli/metabolism
MH  - Middle Aged
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
AID - 0016-5085(88)90421-0 [pii]
AID - 10.1016/0016-5085(88)90421-0 [doi]
PST - ppublish
SO  - Gastroenterology. 1988 Feb;94(2):343-52. doi: 10.1016/0016-5085(88)90421-0.