PMID- 3380099 OWN - NLM STAT- MEDLINE DCOM- 19880725 LR - 20210526 IS - 0270-7306 (Print) IS - 1098-5549 (Electronic) IS - 0270-7306 (Linking) VI - 8 IP - 4 DP - 1988 Apr TI - Amplification of the structurally and functionally altered epidermal growth factor receptor gene (c-erbB) in human brain tumors. PG - 1816-20 AB - By using Southern blot analysis, we found that in two cases of human glioblastoma multiforme, cells carried amplified c-erbB genes which bore short deletion mutations within the ligand-binding domain of the epidermal growth factor (EGF) receptor. The products of these mutated c-erbB genes were about 30 kilodalton (kDa) smaller than the normal 170-kDa EGF receptor, and the tumor cell membrane fractions containing the 140-kDa abnormal EGF receptor showed a significant elevation of tyrosine kinase activity without its ligand. In view of the similarity to the activated viral and cellular erbB genes in the avian system, these mutated and overexpressed EGF receptors might play a role in the onset or development of human glioblastoma cells. FAU - Yamazaki, H AU - Yamazaki H AD - Department of Genetics, University of Tokyo, Japan. FAU - Fukui, Y AU - Fukui Y FAU - Ueyama, Y AU - Ueyama Y FAU - Tamaoki, N AU - Tamaoki N FAU - Kawamoto, T AU - Kawamoto T FAU - Taniguchi, S AU - Taniguchi S FAU - Shibuya, M AU - Shibuya M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Cell Biol JT - Molecular and cellular biology JID - 8109087 RN - 0 (RNA, Messenger) RN - EC 2.7.10.1 (ErbB Receptors) SB - IM MH - Animals MH - Brain Neoplasms/*genetics/metabolism MH - Chromosome Deletion MH - ErbB Receptors/*genetics/metabolism MH - *Gene Amplification MH - *Genes MH - Humans MH - Mice MH - Mice, Nude MH - Mutation MH - Neoplasm Transplantation MH - *Proto-Oncogenes MH - RNA, Messenger/genetics MH - Transplantation, Heterologous MH - Tumor Cells, Cultured/metabolism PMC - PMC363345 EDAT- 1988/04/01 00:00 MHDA- 1988/04/01 00:01 PMCR- 1988/04/01 CRDT- 1988/04/01 00:00 PHST- 1988/04/01 00:00 [pubmed] PHST- 1988/04/01 00:01 [medline] PHST- 1988/04/01 00:00 [entrez] PHST- 1988/04/01 00:00 [pmc-release] AID - 10.1128/mcb.8.4.1816-1820.1988 [doi] PST - ppublish SO - Mol Cell Biol. 1988 Apr;8(4):1816-20. doi: 10.1128/mcb.8.4.1816-1820.1988.