PMID- 3462715
OWN - NLM
STAT- MEDLINE
DCOM- 19861003
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 83
IP  - 17
DP  - 1986 Sep
TI  - Carrier-directed targeting of liposomes and erythrocytes to denuded areas of 
      vessel wall.
PG  - 6603-7
AB  - Immunomorphological staining of specimens prepared from human carotid arteries 
      with anti-collagen type I antibodies reveals large amounts of type I collagen in 
      the subendothelium of lipid fibrous plaques. Collagen type I-containing 
      structures, once in direct contact with blood after plaque rupture, can serve as 
      potential targets for selective delivery of liposomes and erythrocytes to these 
      areas. To verify this rationale, [14C]cholesterol oleate-containing liposomes 
      were conjugated with bovine or human anti-collagen type I antibodies or human 
      plasma fibronectin. Biotin derivatives of human anti-collagen type I antibody 
      were coupled to human erythrocytes. Modified liposomes and erythrocytes were 
      perfused in situ through segments of bovine, rabbit, or human arteries partially 
      denuded with a balloon catheter prior to perfusion. After perfusion, the control 
      and denuded areas were excised and subjected to scanning electron microscopic 
      analysis and measurements of associated radioactivity. It was found that 
      conjugates of liposomes or erythrocytes with anti-collagen type I antibodies or 
      fibronectin are selectively bound by endothelium-free zones of arterial segments. 
      Carrier-directed targeting of drug-laden liposomes and erythrocytes to 
      thrombosis-prone areas of arterial lumen is discussed.
FAU - Smirnov, V N
AU  - Smirnov VN
FAU - Domogatsky, S P
AU  - Domogatsky SP
FAU - Dolgov, V V
AU  - Dolgov VV
FAU - Hvatov, V B
AU  - Hvatov VB
FAU - Klibanov, A L
AU  - Klibanov AL
FAU - Koteliansky, V E
AU  - Koteliansky VE
FAU - Muzykantov, V R
AU  - Muzykantov VR
FAU - Repin, V S
AU  - Repin VS
FAU - Samokhin, G P
AU  - Samokhin GP
FAU - Shekhonin, B V
AU  - Shekhonin BV
AU  - et al.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies)
RN  - 0 (Fibronectins)
RN  - 0 (Liposomes)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Arteriosclerosis/pathology/physiopathology
MH  - Cattle
MH  - Collagen/immunology/*metabolism
MH  - Endothelium/*physiology
MH  - Erythrocyte Membrane/*physiology
MH  - Fibronectins/administration & dosage
MH  - Humans
MH  - In Vitro Techniques
MH  - Liposomes/administration & dosage
PMC - PMC386552
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
PMCR- 1987/03/01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
PHST- 1987/03/01 00:00 [pmc-release]
AID - 10.1073/pnas.83.17.6603 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1986 Sep;83(17):6603-7. doi: 10.1073/pnas.83.17.6603.