PMID- 3477813
OWN - NLM
STAT- MEDLINE
DCOM- 19871104
LR  - 20220317
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 84
IP  - 19
DP  - 1987 Oct
TI  - Increased expression of the epidermal growth factor receptor gene in malignant 
      gliomas is invariably associated with gene amplification.
PG  - 6899-903
AB  - Primary malignant gliomas from 63 patients were analyzed to determine the 
      relationship between amplification of the gene encoding the epidermal growth 
      factor receptor (EGFR) and expression of the corresponding mRNA. Twenty-four 
      tumors were found to have amplified the EGFR gene and amplification of other 
      genes occurred in three additional tumors. Hybridization with synthetic RNA 
      probes was used to quantitate mRNA levels in situ. All 24 tumors with 
      amplification of the EGFR gene had high levels of expression of this gene, while 
      none of the 39 tumors without amplification had increased levels. This shows 
      that, in human gliomas, large increases in the expression of the EGFR gene are 
      invariably associated with alterations in gene structure.
FAU - Wong, A J
AU  - Wong AJ
AD  - Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21205.
FAU - Bigner, S H
AU  - Bigner SH
FAU - Bigner, D D
AU  - Bigner DD
FAU - Kinzler, K W
AU  - Kinzler KW
FAU - Hamilton, S R
AU  - Hamilton SR
FAU - Vogelstein, B
AU  - Vogelstein B
LA  - eng
GR  - CA-09243/CA/NCI NIH HHS/United States
GR  - CA-11998/CA/NCI NIH HHS/United States
GR  - NS-20023/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - *Genes
MH  - Glioma/*genetics/metabolism/pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
MH  - *Oncogenes
MH  - RNA, Messenger/analysis
PMC - PMC299192
EDAT- 1987/10/01 00:00
MHDA- 1987/10/01 00:01
PMCR- 1988/04/01
CRDT- 1987/10/01 00:00
PHST- 1987/10/01 00:00 [pubmed]
PHST- 1987/10/01 00:01 [medline]
PHST- 1987/10/01 00:00 [entrez]
PHST- 1988/04/01 00:00 [pmc-release]
AID - 10.1073/pnas.84.19.6899 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1987 Oct;84(19):6899-903. doi: 10.1073/pnas.84.19.6899.