PMID- 3496132
OWN - NLM
STAT- MEDLINE
DCOM- 19870813
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 70
IP  - 1
DP  - 1987 Jul
TI  - Growth factor requirements of childhood acute leukemia: establishment of 
      GM-CSF-dependent cell lines.
PG  - 192-9
AB  - Eight permanent cell lines were established from cells of 50 consecutive patients 
      with childhood acute leukemia. Three cell lines required growth factor-containing 
      conditioned media. Analysis using blocking antisera and recombinant granulocytic 
      macrophage (GM) colony-stimulating factor (CSF) identified GM-CSF as a growth 
      factor required to establish the latter three cell lines and necessary for their 
      continuous proliferation in chemically defined medium. Two of the 
      GM-CSF-dependent cell lines were derived from patients with undifferentiated T- 
      and a biphenotypic B-myelomonocytic leukemia, which suggests that GM-CSF might 
      maintain proliferation of leukemias originating from immature progenitor cells. 
      Cytogenetic analysis indicated that all established leukemic cell lines were 
      aneuploid, with six lines containing chromosomal alterations related to those 
      observed in the leukemic cells of the patient. Two patients did not have an 
      abnormal clone identified in the marrow but did yield an aneuploid cell line. 
      These studies indicate that GM-CSF-dependent leukemic cell lines can be 
      established in a fraction of childhood leukemia. These cell lines lend themselves 
      to studies aimed at the evaluation in vitro of the role of growth factors in 
      controlling proliferation and differentiation of leukemic cells.
FAU - Lange, B
AU  - Lange B
FAU - Valtieri, M
AU  - Valtieri M
FAU - Santoli, D
AU  - Santoli D
FAU - Caracciolo, D
AU  - Caracciolo D
FAU - Mavilio, F
AU  - Mavilio F
FAU - Gemperlein, I
AU  - Gemperlein I
FAU - Griffin, C
AU  - Griffin C
FAU - Emanuel, B
AU  - Emanuel B
FAU - Finan, J
AU  - Finan J
FAU - Nowell, P
AU  - Nowell P
AU  - et al.
LA  - eng
GR  - CA 10815/CA/NCI NIH HHS/United States
GR  - CA 25875/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Blood Proteins)
RN  - 0 (Culture Media)
RN  - 0 (Interleukin-3)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Blood Proteins/physiology
MH  - Cell Division/drug effects
MH  - *Cell Line
MH  - Child
MH  - Child, Preschool
MH  - Culture Media/physiology
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Interleukin-3/*physiology
MH  - Karyotyping
MH  - Leukemia/blood/*pathology
MH  - Male
MH  - Phenotype
EDAT- 1987/07/01 00:00
MHDA- 1987/07/01 00:01
CRDT- 1987/07/01 00:00
PHST- 1987/07/01 00:00 [pubmed]
PHST- 1987/07/01 00:01 [medline]
PHST- 1987/07/01 00:00 [entrez]
AID - S0006-4971(20)78495-8 [pii]
PST - ppublish
SO  - Blood. 1987 Jul;70(1):192-9.