PMID- 3533968
OWN - NLM
STAT- MEDLINE
DCOM- 19861209
LR  - 20181130
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 32
IP  - 1
DP  - 1986
TI  - Expression of epidermal growth factor receptor and associated glycoprotein on 
      cultured human brain tumor cells.
PG  - 1-10
AB  - The expression of epidermal growth factor (EGF-R) in normal glial and glioma 
      cells grown in culture was examined by using several independent assays. 
      Immunoprecipitation with the monoclonal antibody R1 of extracts from 
      metabolically labeled glial and glioma cells revealed a protein of Mr 
      approximately 170,000, with a migration in sodium dodecyl sulfate-polyacrylamide 
      gels identical to the EGR-R of A431 epidermal carcinoma cells. Furthermore, in 
      the majority of glioma extracts, a protein of Mr approximately 190,000 was 
      specifically immunoprecipitated by this antibody. Similar results were obtained 
      by immunoblotting with a second antibody directed against a synthetic peptide in 
      the sequence of the v-erb-B oncogene. In cell lines expressing both proteins, 
      each was specifically phosphorylated on tyrosine in immune complex kinase assays. 
      The majority of glioma cells bound between 40,000 to 80,000 125I-labeled 
      epidermal growth factor molecules per cell. These results suggest that the 
      expression of EGF-R is common in cultured human glioma cells. In addition, a 
      structurally related protein, is expressed in some of these cells.
FAU - Steck, P A
AU  - Steck PA
FAU - Gallick, G E
AU  - Gallick GE
FAU - Maxwell, S A
AU  - Maxwell SA
FAU - Kloetzer, W S
AU  - Kloetzer WS
FAU - Arlinghaus, R B
AU  - Arlinghaus RB
FAU - Moser, R P
AU  - Moser RP
FAU - Gutterman, J U
AU  - Gutterman JU
FAU - Yung, W K
AU  - Yung WK
LA  - eng
GR  - R01 CA33027/CA/NCI NIH HHS/United States
GR  - R23 CA39803/CA/NCI NIH HHS/United States
GR  - RR5511-23/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Glycoproteins)
RN  - 9004-70-0 (Collodion)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Brain Neoplasms/*metabolism
MH  - Cell Line
MH  - Collodion
MH  - ErbB Receptors/*metabolism
MH  - Glioma/*metabolism
MH  - Glycoproteins/*metabolism
MH  - Humans
MH  - Immunologic Tests
MH  - Immunosorbent Techniques
MH  - Molecular Weight
MH  - Neuroglia/metabolism
MH  - Phosphorylation
MH  - Protein Kinases/metabolism
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
AID - 10.1002/jcb.240320102 [doi]
PST - ppublish
SO  - J Cell Biochem. 1986;32(1):1-10. doi: 10.1002/jcb.240320102.