PMID- 3681981
OWN - NLM
STAT- MEDLINE
DCOM- 19880107
LR  - 20190710
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 196
IP  - 4
DP  - 1987 Aug 20
TI  - Canonical structures for the hypervariable regions of immunoglobulins.
PG  - 901-17
AB  - We have analysed the atomic structures of Fab and VL fragments of immunoglobulins 
      to determine the relationship between their amino acid sequences and the 
      three-dimensional structures of their antigen binding sites. We identify the 
      relatively few residues that, through their packing, hydrogen bonding or the 
      ability to assume unusual phi, psi or omega conformations, are primarily 
      responsible for the main-chain conformations of the hypervariable regions. These 
      residues are found to occur at sites within the hypervariable regions and in the 
      conserved beta-sheet framework. Examination of the sequences of immunoglobulins 
      of unknown structure shows that many have hypervariable regions that are similar 
      in size to one of the known structures and contain identical residues at the 
      sites responsible for the observed conformation. This implies that these 
      hypervariable regions have conformations close to those in the known structures. 
      For five of the hypervariable regions, the repertoire of conformations appears to 
      be limited to a relatively small number of discrete structural classes. We call 
      the commonly occurring main-chain conformations of the hypervariable regions 
      "canonical structures". The accuracy of the analysis is being tested and refined 
      by the prediction of immunoglobulin structures prior to their experimental 
      determination.
FAU - Chothia, C
AU  - Chothia C
AD  - MRC Laboratory of Molecular Biology, Cambridge, England.
FAU - Lesk, A M
AU  - Lesk AM
LA  - eng
GR  - GM25435/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Immunoglobulin Variable Region)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites, Antibody
MH  - *Immunoglobulin Variable Region
MH  - Models, Molecular
MH  - Protein Conformation
MH  - Surface Properties
EDAT- 1987/08/20 00:00
MHDA- 1987/08/20 00:01
CRDT- 1987/08/20 00:00
PHST- 1987/08/20 00:00 [pubmed]
PHST- 1987/08/20 00:01 [medline]
PHST- 1987/08/20 00:00 [entrez]
AID - 0022-2836(87)90412-8 [pii]
AID - 10.1016/0022-2836(87)90412-8 [doi]
PST - ppublish
SO  - J Mol Biol. 1987 Aug 20;196(4):901-17. doi: 10.1016/0022-2836(87)90412-8.