PMID- 3701929
OWN - NLM
STAT- MEDLINE
DCOM- 19860624
LR  - 20220310
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 58
IP  - 3
DP  - 1986 Jun
TI  - Pathogenicity of antigenic variants of murine coronavirus JHM selected with 
      monoclonal antibodies.
PG  - 869-75
AB  - To analyze the pathogenesis of the neurotropic murine coronavirus JHMV, we used 
      monoclonal antibodies to the E2 viral glycoprotein to select antigenic variant 
      viruses. Monoclonal antibodies J.7.2 and J.2.2 were shown to bind to 
      topographically distinct regions of the E2 molecule, and the variants selected 
      with the two antibodies demonstrated very different disease pictures in mice. 
      Variants selected with J.7.2 were, like the parental virus, highly virulent and 
      caused an acute encephalitic illness. By contrast, J.2.2-selected variants 
      predominantly caused a subacute paralytic disease clinically and extensive 
      demyelination histologically. Antigenic differences among the variants and 
      parental virus were readily demonstrable with anti-E2 monoclonal antibodies. 
      However, no differences between the viruses could be shown in binding studies 
      with monoclonal antibodies directed against either E1 or N, the other two JHMV 
      structural proteins. Since only J.2.2 selected demyelinating variants with 
      reduced neurovirulence, it is likely that this monoclonal antibody recognizes a 
      subregion of the E2 molecule that is particularly important in JHMV pathogenesis.
FAU - Fleming, J O
AU  - Fleming JO
FAU - Trousdale, M D
AU  - Trousdale MD
FAU - el-Zaatari, F A
AU  - el-Zaatari FA
FAU - Stohlman, S A
AU  - Stohlman SA
FAU - Weiner, L P
AU  - Weiner LP
LA  - eng
GR  - NS00795/NS/NINDS NIH HHS/United States
GR  - NS07149/NS/NINDS NIH HHS/United States
GR  - NS18146/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antigens, Viral/*analysis
MH  - Coronaviridae/growth & development/immunology/*pathogenicity
MH  - Demyelinating Diseases/etiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Paralysis/etiology
MH  - Virulence
MH  - Virus Replication
PMC - PMC252994
EDAT- 1986/06/01 00:00
MHDA- 1986/06/01 00:01
PMCR- 1986/06/01
CRDT- 1986/06/01 00:00
PHST- 1986/06/01 00:00 [pubmed]
PHST- 1986/06/01 00:01 [medline]
PHST- 1986/06/01 00:00 [entrez]
PHST- 1986/06/01 00:00 [pmc-release]
AID - 10.1128/JVI.58.3.869-875.1986 [doi]
PST - ppublish
SO  - J Virol. 1986 Jun;58(3):869-75. doi: 10.1128/JVI.58.3.869-875.1986.