PMID- 3802388
OWN - NLM
STAT- MEDLINE
DCOM- 19870220
LR  - 20190511
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 8
IP  - 1
DP  - 1987 Jan
TI  - Inhibition of carcinogen-altered rat tracheal epithelial cell proliferation by 
      normal epithelial cells in vivo.
PG  - 145-50
AB  - The experiments described investigate the potential influence of surrounding 
      normal tracheal epithelial cells on the survival and growth of carcinogen-exposed 
      epithelial cells in tracheal mucosa reconstructed from known cell mixtures. Cell 
      mixtures containing preneoplastic or neoplastic rat tracheal epithelial cells and 
      a small fraction of normal tracheal or esophageal epithelial cells were 
      inoculated into the lumen of previously frozen-thawed tracheas which were then 
      transplanted s.c. into syngeneic hosts. Within 2-3 weeks an intact tracheal 
      mucosa was regenerated from the inoculated cells. At various times after cell 
      inoculation and transplantation, cells were harvested from the repopulated 
      trachea and the number of diploid normal and/or aneuploid carcinogen-exposed 
      tracheal epithelial cells determined by flow cytometry (DNA content) and the 
      frequency of cells with altered in vitro growth capacity determined. The data 
      suggest that normal tracheal epithelial cells have an enhanced 'survival' 
      capacity relative to carcinogen-exposed cell lines in the regenerated tracheal 
      mucosa. When greater than 10(4) normal cells were inoculated with a 2- to 
      100-fold excess of carcinogen-exposed cell lines the regenerated epithelium was 
      comprised almost entirely of normal-diploid-epithelial cells. Independent of the 
      ratio of normal to altered cells, when less than 10(4) normal epithelial cells 
      were inoculated in the mixture some carcinogen-altered cells and some normal 
      cells were present in the regenerated epithelium. When established repopulated 
      tracheas containing mixtures of normal and neoplastic epithelial cells were left 
      in the animal no tumors developed. In contrast tracheas containing neoplastic 
      cells alone or partially scraped tracheas containing comparable numbers of 
      neoplastic cells covering a contiguous area of the submucosa, tumors developed 
      within 2-5 weeks of cell inoculation and transplantation. These data suggest that 
      normal tracheal epithelial cells have the capacity to modulate expression of the 
      neoplastic phenotype (tumor development) in neoplastic populations. This effect 
      appears to require close contact with the normal cells as inhibition is not 
      observed when the neoplastic cells occupy a single contiguous area of the 
      trachea.
FAU - Terzaghi-Howe, M
AU  - Terzaghi-Howe M
LA  - eng
GR  - 5R01 CA34695/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Carcinogens)
SB  - IM
MH  - Animals
MH  - Carcinogens/*pharmacology
MH  - *Cell Communication
MH  - Cell Division/drug effects
MH  - *Epithelial Cells
MH  - Epithelium/drug effects
MH  - Female
MH  - Neoplasm Transplantation
MH  - Phenotype
MH  - Precancerous Conditions/pathology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Trachea/*cytology/drug effects
MH  - Tracheal Neoplasms/pathology
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1093/carcin/8.1.145 [doi]
PST - ppublish
SO  - Carcinogenesis. 1987 Jan;8(1):145-50. doi: 10.1093/carcin/8.1.145.