PMID- 6033532
OWN - NLM
STAT- MEDLINE
DCOM- 19671007
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 34
IP  - 1
DP  - 1967 Jul
TI  - Fine structural localization of a blood-brain barrier to exogenous peroxidase.
PG  - 207-17
AB  - Horseradish peroxidase was administered to mice by intravenous injection, and its 
      distribution in cerebral cortex studied with a recently available technique for 
      localizing peroxidase with the electron microscope. Brains were fixed by either 
      immersion or vascular perfusion 10-60 min after administration of various doses 
      of peroxidase. Exogenous peroxidase was localized in the lumina of blood vessels 
      and in some micropinocytotic vesicles within endothelial cells; none was found 
      beyond the vascular endothelium. Micropinocytotic vesicles were few in number and 
      did not appear to transport peroxidase while tight junctions between endothelial 
      cells were probably responsible for preventing its intercellular passage. Our 
      findings therefore localize, at a fine structural level, a "barrier" to the 
      passage of peroxidase at the endothelium of vessels in the cerebral cortex. The 
      significance of these findings is discussed, particularly with reference to a 
      recent study in which similar techniques were applied to capillaries in heart and 
      skeletal muscle.
FAU - Reese, T S
AU  - Reese TS
FAU - Karnovsky, M J
AU  - Karnovsky MJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - EC 1.11.1.- (Peroxidases)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/*physiology
MH  - Cerebral Cortex/*metabolism
MH  - Histocytochemistry
MH  - Mice
MH  - Microscopy, Electron
MH  - Peroxidases/*pharmacology
MH  - Pinocytosis
PMC - PMC2107213
EDAT- 1967/07/01 00:00
MHDA- 1967/07/01 00:01
PMCR- 1968/01/01
CRDT- 1967/07/01 00:00
PHST- 1967/07/01 00:00 [pubmed]
PHST- 1967/07/01 00:01 [medline]
PHST- 1967/07/01 00:00 [entrez]
PHST- 1968/01/01 00:00 [pmc-release]
AID - 10.1083/jcb.34.1.207 [doi]
PST - ppublish
SO  - J Cell Biol. 1967 Jul;34(1):207-17. doi: 10.1083/jcb.34.1.207.