PMID- 6203040
OWN - NLM
STAT- MEDLINE
DCOM- 19840717
LR  - 20220316
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 309
IP  - 5968
DP  - 1984 Jun 7-13
TI  - Inability of Rous sarcoma virus to cause sarcomas in the avian embryo.
PG  - 552-6
AB  - The injection of Rous sarcoma virus (RSV) into the wing web of newly hatched 
      chicks causes a rapidly growing sarcomatous tumour which is palpable within 1 
      week of inoculation; and cultures of fibroblasts derived from chick embryos (CEF) 
      and infected with RSV become rapidly transformed. Genetic studies have determined 
      that expression of a single viral gene, designated v-src, is necessary for 
      neoplastic transformation. This gene codes for a 60,000-molecular weight 
      phosphoprotein termed pp60SPC , which possesses a protein kinase activity that 
      phosphorylates polypeptides on tyrosine residues and is constitutively expressed 
      in infected CEF cells. It has been suggested that transformation, and possibly 
      tumorigenesis, may result solely from the consequences of this increase in 
      tyrosine phosphorylations. The pathogenicity of RSV in chick embryos in ovo is 
      less clear. Murphy and Rous suggested that RSV may have caused tumours in 
      "various tissues" of "some embryos", but the subsequent studies of Milford and 
      Duran - Reynals , as well as several other laboratories, failed to find any 
      evidence of intraembryonic tumours in RSV-infected early embryos. The findings of 
      Duran - Reynals , if correct, cannot be explained easily in view of our present 
      understanding of RSV tumorigenicity. Thus, we have re-examined the interaction of 
      RSV with the avian embryo and confirm here that RSV is nontumorigenic and 
      non-teratogenic when microinjected into day 4 chicken embryos. In addition, we 
      found that (1) the virus not only replicates in the embryo, but it also expresses 
      an active src-specific protein kinase and (2) once the cells from the infected 
      limbs are disrupted and placed in culture, they are capable of expressing the 
      transformed phenotype after a 24-h delay.
FAU - Dolberg, D S
AU  - Dolberg DS
FAU - Bissell, M J
AU  - Bissell MJ
LA  - eng
GR  - 1 F32 CA 07068-01A1/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Avian Sarcoma Viruses/enzymology/*pathogenicity
MH  - *Cell Transformation, Neoplastic
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Fibroblasts/physiology
MH  - Protein Kinases/metabolism
MH  - RNA-Directed DNA Polymerase/metabolism
MH  - Sarcoma, Experimental/*microbiology
MH  - Time Factors
EDAT- 1984/06/07 00:00
MHDA- 1984/06/07 00:01
CRDT- 1984/06/07 00:00
PHST- 1984/06/07 00:00 [pubmed]
PHST- 1984/06/07 00:01 [medline]
PHST- 1984/06/07 00:00 [entrez]
AID - 10.1038/309552a0 [doi]
PST - ppublish
SO  - Nature. 1984 Jun 7-13;309(5968):552-6. doi: 10.1038/309552a0.