PMID- 6276390
OWN - NLM
STAT- MEDLINE
DCOM- 19820420
LR  - 20220330
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 257
IP  - 3
DP  - 1982 Feb 10
TI  - A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma 
      membrane vesicles.
PG  - 1523-31
AB  - A method is presented for the preparation of a "native" epidermal growth factor 
      (EGF) receptor-kinase complex of molecular weight 170,000 from A-431 cells. 
      Although this receptor complex is capable of binding EGF, noncovalently, in 
      quantities similar to the previously isolated 150,000 complex (Cohen, S., 
      Carpenter, G., and King, L., Jr. (1980) J. Biol. Chem. 255, 4834-4842), the 
      170,000 preparation has 5 to 10 times the intrinsic kinase activity 
      (autophosphorylation). However, the 170,000 kinase activity toward other proteins 
      is lower than that of the 150,000 preparation. Both the 170,000 and 150,000 
      kinase activities are enhanced by EGF. The 170,000 and 150,000 proteins are also 
      capable of forming covalent linkages to 125I-EGF, and each is precipitated by 
      antisera directed against the 170,000 protein. We suggest the 150,000 protein is 
      a proteolytic degradation product of the 170,000 protein. The EGF-enhanced kinase 
      activity of the 170,000 preparation remains associated with the 125I-EGF-binding 
      activity following EGF affinity chromatography, electrophoresis in nondenaturing 
      gels, or immunoprecipitation with antisera directed against the sodium dodecyl 
      sulfate (SDS) gel-purified 170,000 protein. These results indicate that the 
      receptor, kinase, and substrate domains are linked, possibly covalently.
FAU - Cohen, S
AU  - Cohen S
FAU - Ushiro, H
AU  - Ushiro H
FAU - Stoscheck, C
AU  - Stoscheck C
FAU - Chinkers, M
AU  - Chinkers M
LA  - eng
GR  - HD-00700/HD/NICHD NIH HHS/United States
GR  - HD-07043/HD/NICHD NIH HHS/United States
GR  - RR-05424/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Receptors, Cell Surface)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/metabolism/ultrastructure
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epidermal Growth Factor/metabolism
MH  - ErbB Receptors
MH  - Kinetics
MH  - Microscopy, Electron, Scanning
MH  - Molecular Weight
MH  - Protein Kinases/*isolation & purification
MH  - Receptors, Cell Surface
EDAT- 1982/02/10 00:00
MHDA- 1982/02/10 00:01
CRDT- 1982/02/10 00:00
PHST- 1982/02/10 00:00 [pubmed]
PHST- 1982/02/10 00:01 [medline]
PHST- 1982/02/10 00:00 [entrez]
AID - S0021-9258(19)68224-4 [pii]
PST - ppublish
SO  - J Biol Chem. 1982 Feb 10;257(3):1523-31.