PMID- 6286115
OWN - NLM
STAT- MEDLINE
DCOM- 19821012
LR  - 20240104
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 42
IP  - 9
DP  - 1982 Sep
TI  - Growth of human hepatoma cells lines with differentiated functions in chemically 
      defined medium.
PG  - 3858-63
AB  - A human hepatoma cell line, HuH-7, which was established from a hepatocellular 
      carcinoma, was found to replicate continuously in a chemically defined medium 
      when the medium was supplemented with Na2SeO3. The cells grew better in this 
      medium than in serum-containing medium without any adaptation period. Other 
      established human hepatoma and hepatoblastoma cell lines, HuH-6 cl-5, PLC/PRF/5, 
      huH-1, and huH-4, also grew in the defined medium. Although HLEC-1 cells failed 
      to proliferate continuously with Na2SeO3 alone, they grew if a cell-free 
      conditioned medium from HuH-7 cells was added to the medium. These cell lines, 
      except the HLEC-1 cell line, produced the following human plasma proteins among 
      those examined: albumin, prealbumin, alpha 1-antitrypsin, ceruloplasmin, 
      fibrinogen, fibronectin, haptoglobin, hemopexin, beta-lipoprotein, alpha 
      2-macroglobulin, beta 2-microglobulin, transferrin, lipoprotein, alpha 
      2-macroglobulin, beta 2-microglobulin, transferrin, Complement Components 3 and 
      4, and alpha 1-fetoprotein. Beside plasma proteins, the media from HuH-7, HuH-6 
      cl-5, PLC/PRF/5, and huH-1 contained anti-carcinoembryonic antigen-reactive 
      proteins, and those from PLC/PRF/5, huH-1, and huH-4 medium contained hepatitis B 
      surface antigen. These proteins were detected during periods of serial 
      cultivation over 9 months under the above culture conditions. The hepatoma cell 
      lines grown in the fully defined synthetic medium may provide a new approach for 
      investigating the growth and metabolism of human hepatoma cells in vitro.
FAU - Nakabayashi, H
AU  - Nakabayashi H
FAU - Taketa, K
AU  - Taketa K
FAU - Miyano, K
AU  - Miyano K
FAU - Yamane, T
AU  - Yamane T
FAU - Sato, J
AU  - Sato J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Blood Proteins)
RN  - 0 (Culture Media)
RN  - 0 (Selenium Compounds)
RN  - 0 (Selenium Oxides)
RN  - 9013-90-5 (Lactalbumin)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Blood
MH  - Blood Proteins/biosynthesis
MH  - Carbohydrate Metabolism
MH  - Carcinoma, Hepatocellular/metabolism/*pathology
MH  - Cell Division
MH  - *Cell Line
MH  - Culture Media
MH  - Humans
MH  - Karyotyping
MH  - Kinetics
MH  - Lactalbumin
MH  - Liver Neoplasms
MH  - Selenium/pharmacology
MH  - *Selenium Compounds
MH  - Selenium Oxides
EDAT- 1982/09/01 00:00
MHDA- 1982/09/01 00:01
CRDT- 1982/09/01 00:00
PHST- 1982/09/01 00:00 [pubmed]
PHST- 1982/09/01 00:01 [medline]
PHST- 1982/09/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1982 Sep;42(9):3858-63.